PuysioLocicaL AcTION oF NITROBENZENE VAPOR ON ANIMALS 455 
COCCIDIA 
Specimens of Eimeria avium in the odcyst stage were recovered from 
the feces of Hen VII. These odcysts appeared normal, and the develop- 
ment of sporocysts and sporozoites occurred as usual. 
EXPERIMENT TO DETERMINE THE ACTION OF NITROBENZENE ON 
DIGESTIVE FUNCTIONS 
Post-mortem examinations of animals poisoned by nitrobenzene usually 
revealed the fact that food which had been in the stomach for some days 
had not started to digest. A special experiment was therefore conducted 
in order to check these observations. 
At 11 a. m. on October 3, 1916, four kittens, all from the same litter, 
were each given an equally large portion of boiled hamburg steak. At 
11.10 three of these kittens were placed in the fumigation chamber, where 
they were fumigated for four hours at a temperature of 22° C. 
At 2.20 p. m. (three hours and ten minutes after the fumigation was 
begun) one of the animals died. The other two were removed from the 
tank at 3.10 and showed well-advanced symptoms of poisoning. One 
of them died at about 4 p. m. and the other at about 5 p. m. 
At about 5.30 p. m. the control kitten was killed with chloroform and 
a post-mortem examination was made of all four animals. The stomachs 
of the animals that had been fumigated contained the full amount of 
the hamburg steak eaten, and in no case had it even started to digest. 
The digestion of the hamburg steak in the stomach of the control animal 
was well advanced. 
EXPERIMENTS TO DETERMINE THE ACTION OF NITROBENZENE ON BLOOD 
BLOOD COUNTS 
Two pups, from the same litter and about three months old, were 
kept under similar conditions,” and blood counts were made on each for 
several days in order to determine the normal counts. Each animal was 
then fumigated, on different days and for different periods of time, and the 
blood counts were continued. Always, of course, as soon as an animal 
developed pronounced symptoms of poisoning it refused food, and this 
interfered with exact comparisons. The results of these experiments 
are indicated in the following tables: 
12 These animals were fed twice a day, each receiving at a meal 40 grams of cooked liver, + pint of milk, 
50 grams of bread, and all the water it wanted. 
