PuystoLoercaL ACTION OF NITROBENZENE VAPOR ON ANIMALS 461 
The Purkinje cells from Dog F (Plate VI, 2) were greatly swollen, and 
the Nissl bodies (tigroid bodies) were almost entirely absent. There was 
no stainable substance near the periphery, and that around the nucleus 
was massed and indefinite. Some of the Purkinje cells from Dog E 
were like those from Dog F; others were very much shrunken, about 
one-half “normal” size, and the whole was a darkly stained, indefinite 
mass with the nucleus almost obliterated. The Purkinje cells from Dog G 
were all very much shrunken (Plate VI, 4), and no Nissl bodies were to 
be seen. There was a small amount of stainable substance, massed and 
clinging about a much-shrunken nucleus. In many cases the nucleus 
had disappeared, and in numerous instances the entire cell seems to have 
disappeared. The Purkinje cells from the control animal, Dog H, were 
all apparently normal, the tigroid bodies staining excellently (Plate VI, 1). 
The above experiment was repeated, and this time two controls were 
used instead of one. One of the poisoned animals was killed shortly 
after the first appearance of the symptoms, and only a few of the Purkinje 
cells from this animal showed degeneration. Another one of the animals 
was killed before the symptoms were very far advanced, and in this case 
but few of the Purkinje cells looked wholly normal and some of them 
showed definite chromatolytic degeneration. The third poisoned animal 
was killed after the symptoms were well advanced, and the cells from this 
animal had become so thoroly degenerated that there was scarcely any- 
thing left of them but irregular blotches. The Purkinje cells from the 
control animals were all apparently normal. 
Sections were made, stained, and mounted on the same slide, of the 
following additional tissues from the control animals and from the animal 
in this later experiment in which the symptoms of poisoning were well 
advanced: liver, spleen, thyroid, adrenal gland, duodenum, and rectus 
femoris muscle. No signs of degeneration could be observed in any 
of these tissues, but there appeared to be a slight hyperemia of the liver, 
the duodenum, and the rectus femoris muscle; these tissues were dissected 
out before the infiltration of the saline solution, so that the blood remained 
in them. 
BIRDS 
Two pigeons were fumigated with nitrobenzene, and when the symptoms 
became pronounced, they, together with a control, were killed by clipping 
