495 
A. B. BEAUMONT 
a water extract of the material was used as a check. Results are given 
in table 4: 
TABLE 4. ApsorptTion oF Dyrres By Lower Forms oF Puant Lire 
(C-C:) -dye | 
(C-C:) :dye adsorbed by 
Dye used adsorbed by | water extract | Difference 
water extract plus fungus 
or alga 
(a) A fungus (Botrytis) 
Methylenetbluemare sarerias ion ane eee 78.7+0.2 91.90.16 | 1322220-26 
Diaminersky=luepmy re eee erie eerie 83.6+£0.5 92.60.22 9.00.55 
Malla chitesoneem sys 0g pen sere are eee 80.0++0.0 95.8+0.06 | 15.8+0.06 
(b) An alga (Cladophora) 
Methyleneibluevs ehcp ee aren: 797 .9+3.3 993.8+0.03 | 195.9=+-3.3 
Miamineysky-pluew sia. ace eee seen 824.5+2.5 960. 03-- a7 Nels omosae os 
Mialachitesoneemsra: rt cine aes oe 877 .2+2.0 933 .3+1.87 56.1+2.7 
* No probable error. Cloudy. Diificult to read. 
The differences are significant, and from them it is evident that both 
alga and fungus adsorb dye. The alga was found to be very adsorptive. 
Therefore, in dye-adsorption experiments where there is a chance for 
these organisms to grow, they should be taken into consideration. 
The point just made is further substantiated by the data presented in 
table 5. The treatment of the soils was the same except for sterilization. 
They were allowed to remain moist for several months after sterilization 
and before the dye-adsorption experiment. 
TABLE 5. Errsecr oF Previous STERILIZATION ON THE ADSORPTION OF METHYLENE 
BuiuE BY DUNEIRK SURFACE SOIL 
| 
(C-C,) -dy 
wae aa Difference 
Sterilized, no growth apparent........... 82.25+0.38 2 
aa Ol 7a==0red 
Not sterilized, growth apparent...:......| 89.00+0.03 
Methods used in this work 
At the outset the writer was of the opinion that some of the colloidal 
states could best be measured by certain methods and other states by 
