734 CHARLES PauL ALEXANDER 
The more important recorded protozoan parasites are as follows: 
Class, Sporozoa. 
Subclass, Telosporidia. 
Order, Coccidiomorpha. 
Family, Adeleidae. 
1. Adelea tipulae Léger. In the intestine of species of Tipula. 
Order, Gregarinida. 
Family, Gregarinidae. 
2. Hirmocystis polymorpha (Léger, 1892:113). In the intestine of the larva of Limnobia sp. 
3. H. ventricosa (Léger, 1892:111). In the alimentary canalof Tipula oleracea, Nephrotoma 
pratensis, and other species. 
. Gregarina longa (Léger, 1892:117). In the alimentary canal of a species of Tipula. 
Family, Actinocephalidae. 
Actinocephalus tipulae Léger (1892:141). In the alimentary canal of Tipula larvae. 
Probably the same species has been recorded from the larvae of a species of Ctenophora. 
6. Pileocephalus striatus Léger & Duboseq (1909:887—-893). In the mid-intestinal epithelium 
of the larva of Ptychoptera contaminata. The Pileocephalus live in the epithelium of the 
mid-intestine, attaching themselves to the epithelial cells and hypertrophying the adjoin- 
ing tissues. They obtain their nutriment from the food that penetrates into the <ells. 
Family, Stylorhynchidae. 
7. Near Stylorhynchus (Miall, 1893:237). In the stomach of larvae of Dicranota bimaculata. 
Subclass, Neosporidia. 
Order, Cnidosporidia. 
Suborder, Microsporidia. 
Family, Nosematidae. 
8. Nosema strictum Monz. (Moniez, 1887). In muscles, conjunctive tissue, and other parts 
of Nephrotoma pratensis. 
9. Gurleya francottei Léger & Duboseq (1909:894). In the epithelium of the mid-intestine 
of the larva of Ptychoptera contaminata. 
Class, Flagellata. 
Family, Trypanosomidae (Herpetomonadidae). 
10. Crithidia campanulata Léger. At the juncture of the mid- and hind-intestines in the 
larva of Ptychoptera contaminata (Léger & Duhoscq, 1909:898-900). 
iN 
on 
The writer is indebted to Dr. R. Kudo for assistance in determining the 
terminology used above. 
Bacteria are frequently found in crane-fly larvae. Léger and Duboseq 
(1909:900-901) record undetermined spirochaetes in the epithelial cells 
of the posterior part of the mid-intestine of Ptychoptera contaminata. 
Dr. Hugh Glasgow, of the Geneva Experiment Station, informs the writer 
that in Illinois a large tipulid larva, probably that of Tzpula abdominalzs, 
living in the leaf-drift of prairie streams, is heavily infested with bacteria. 
Most of the specimens observed had an abundance of small coccus and 
spirochaete forms, with occasional specimens of a gigantic bacillus measur- 
ing from forty to eighty microns in length and disporous. These large 
bacilli infest the hind-gut of the larva. 
