TREATMENT OF TRYPANOSOMIASIS. 39 



surra. Blood examination April 30 was positive for trypanosomata. On this 

 date 8 grams of arsacetin were given subeutaneously. The parasites disappeared 

 from the animal's blood and remained absent. On May 5 there was given a 

 second inoculation of 15 grams of arsacetin intravenously. The horse died on 

 the following day of arsenic poisoning. A monkey inoculated on May 6 with 20 

 cubic centimeters of its blood did not develop surra. 



Horse No. 8. — Chinese horse, infected artificially in the laboratory. Inoculated 

 subeutaneously with 10 cubic centimeters of the blood of horse No. 2 just before 

 it died of surra. Horse No. 8 was untreated and died of surra on June 15. On 

 June 4, June 14, and May 17, respectively, monkeys were inoculated with the blood 

 of this horse and all developed surra infection and died in nineteen, and seven- 

 teen, and eleven days, respectively, thus demonstrating the virulence of the strain 

 employed in these experiments. 



Horse No. 0. — Small native horse; infected naturally with surra. No informa- 

 tion regarding the length of time of infection. On May 21 the animal was injected 

 with 10 grams of arsenophenylglycin intravenously. Trypanosomata disappeared 

 from the blood. On June 12 the animal's temperature rose to 39.8° although 

 no parasites could be found in the blood. A monkey was inoculated with 20 

 cubic centimeters of the blood and on June 22 was found to have trypanosomata 

 in its blood. Because of the rise of temperature on June 12, the horse was 

 given another injection of 10 grams of arsenophenylglycin intravenously. On 

 June 27, the horse was unable to rise, although the blood showed no try- 

 panosomata. On June 28 a monkey was inoculated with 12 cubic centimeters 

 of the horse's blood. This monkey, however, did not develop trypanosomiasis. 

 The horse died on June 30, the temperature being 40°. This animal also probably 

 died of surra infection. 



Horse No. 10. — Native horse; contracted the disease naturally. (Edema of 

 the abdomen and scrotum at the time the treatment was begun. No informa- 

 mation regarding the length of time since the animal contracted the infection. 

 On May 21 it was given an injection of 10 grams of arsenophenylglycin. Try- 

 panosomata disappeared from the blood. On June 7, oedema of the abdomen 

 and scrotum was still present. A monkey was inoculated with 35 cubic centi- 

 meters of the blood of this horse and died on June 18 of surra. On June 10, 

 the trypanosomata reappeared in the horse's blood, when 10 grams of arsenophe- 

 nylglycin were given intravenously. On June 29 another monkey was inoculated 

 with 22 cubic centimeters of the horse's blood. This monkey died later of surra. 



On June 29 the horse was given 10 grams of arsenophenylglycin subeuta- 

 neously and on July 14 he was unable to rise. On July 16 the horse was killed. 

 The examination of the blood and organs were negative for trypanosomata. This 

 animal probably also had surra infection when it was killed. 



Horse No. 11. — Native pony; contracted the disease naturally. No informa- 

 tion as to length of time the animal had been infected. (Edema of the abdomen 

 and other symptoms of surra present. This animal was not treated and died 

 on June 1 of surra, the animal being used for control purposes. 



Horse No. 12. — Arrived in the advanced stages of surra. The animal was 

 killed on May 12, but no trypanosomata were found in the spleen, bone marrow, 

 lymphatic glands, or blood. Fluid from the lateral ventricle of the brain was 

 collected and eentrifugated. A few trypanosomata were found in the sediment. 



Horse No 13. — Large American horse; infected artificially with 1 cubic centi- 

 meter of the blood of a monkey infected with surra. This monkey had been 

 infected six days previously with the blood of horse No. 8. Five days later the 

 blood of the horse showed numerous trypanosomata. This animal was untreated 

 and died of surra on July 14, less than one month from the date of its infection. 



