TREATMENT OF TRYPANOSOMIASIS. 41 



the animal was found dead on tbe morning- of November 4, evidently having 

 succumbed to arsenic poisoning. 



Horse No. 21. — American horse (weight 521 kilos) ; injected with 5 cubic 

 centimeters of the blood of horse No. 19 on October 19. October 27 positive for 

 trypanosomata. Thirty grams of arsenoplienylglycin were given by subcutaneous 

 and intravenous injection (0.058 gram per kilo). There was profuse perspiration 

 following the injection and the animal became very restless. On November 

 a considerable area of induration appeared just above the front shoulders of 

 the horse. This was incised but no pus was present. On November 12, 10 cubic 

 centimeters of the blood of the horse were injected into a monkey. This monkey 

 later developed surra on November 19, from which it died on November 24. 

 On November 12, although the condition of the animal appeared good, 20 grams 

 of arsenoplienylglycin were again injected _ intravenously. On November 29, 

 although the blood of the horse was negative for parasites by microscopical 

 examination, a second monkey was inoculated with its blood. This animal devel- 

 oped surra infection on December 6. On November 29, the horse was given 19 

 grams of arsenoplienylglycin intravenously. On December 7 the blood of the 

 horse was again injected into another monkey. This animal remained negative 

 for parasites. December 7 the horse was given another injection of 20 grams of 

 arsenoplienylglycin intravenously. 



On January 3 the temperature registered 40°. An examination of the blood 

 showed a few trypanosomata present. Twenty-five grams of the drug were then 

 given intravenously. The parasites then disappeared from the blood and were 

 not found present on repeated examinations up to January 20. On January 19 

 the condition of the horse appeared to be good. On January 20, although his 

 temperature was normal, he refused to eat and became unable to rise and died 

 a few hours later. The autopsy showed advanced glomerular nephritis, cloudy 

 swelling of the liver and heart muscle. The large intestine was greatly distended 

 with gas. The spleen was somewhat soft and friable. Microscopic examination 

 of the blood was negative after repeated examinations. The cerebral fluid from 

 the ventricles and the spinal fluid were centrifugated and one trypanosoma was 

 found in each of three smears made from the sediment. A monkey was then 

 inoculated with some of the blood and later developed surra. 



The treatment of this horse demonstrates very forcibly the inefficiency 

 of the drug in some instances. This animal had received five intravenous 

 injections of arsenoplienylglycin in amounts of 30, 20, 19, 20 and 

 25 grams. Notwithstanding the fact that at autopsy marked lesions due 

 to arsenic poisoning were present, nevertheless a few trypanosomata had 

 resisted the action of the drug and these were active and proved capable 

 of causing infection and death in another animal. Furthermore, the 

 treatment was begun in this horse eight clays after infection. 



Horse No. 25. — American horse, brought to laboratory on February 1 for 

 treatment for surra. At the time of its arrival the disease was evidently well 

 advanced. A blood examination showed the presence of trypanosomata and of 

 microfilaria?. Nineteen grams of arsenoplienylglycin were injected intravenously. 

 On the following day there was considerable swelling about the point of the in- 

 jection. The animal gradually grew r worse, the cedema increased, and it died 

 on February 15. Three days before its death there was considerable fever, but 

 the blood was negative for trypanosomata. 



Mule {American) No. 26. — This animal was observed in the Province of Bu- 

 lacan. The blood was examined on December 19 and trypanosomata found therein. 



