TREATMENT OF TRYPANOSOMIASIS. 43 



This animal is apparently well. Bepeated examinations of its blood 

 and inoculations of it into 3 monkeys have been made. None of the 

 animals have developed trypanosomiasis. 



Horse No. 2J(. — American colt infected artificially with 5 cubic centimeters of 

 the blood of horse No. 22 on December 4. December 7 blood examination showed 

 a few trypanosomata. December 9, trypanosomata numerous, when 10 grams 

 of arsenic sulphide were administered by the mouth in capsules. December 10, 

 trypanosomata numerous; December 11, 5 grams of arsacetin injected subcu- 

 taneously. From December 11 to December 17 the horse was given alternate 

 doses of arsenic sulphide and arsacetin and died on December IS of arsenic 

 poisoning. 



Mule (American) No. 27. — This animal was also examined at Bulacan on 

 December 17 and trypanosomata found in its blood. On the same date 15 

 grams of arsenic sulphide were given by mouth. December 19 the blood was 

 still positive for parasites and 15 grams of arsenophenylglycin were injected 

 intravenously. The animal was brought to the laboratory on December 21. Its 

 temperature on examination was 40° and there was considerable oedema between 

 the front shoulders. The temperature dropped to normal' on December 24, 

 although the marked oedema increased along the whole of the abdomen. Decem- 

 ber 27, 20 grams of arsenophenylglycin were given intravenously, although the 

 blood remained negative for parasites. Twenty-five grams of the drug were 

 again given on January 4 and 25 grams on January 25. A monkey was in- 

 oculated on January 25 just before the injection of the drug and did not develop 

 surra infection. On February 14, 10 grams of arsenic sulphide were given the 

 animal by the mouth, through a mistake on the part of the attendant. On 

 March 23 trypanosomata reappeared in his blood and he was given 30 grams of 

 arsenophenylglycin. 



This animal is at present alive and its blood contains no trypanoso- 

 mata. It seems not unlikely that this animal was reinfected with surra 

 from another mule which was suffering with the disease and which was 

 kept with it. 



Mule [American) No. 28. — This mule was found positive for trypanosomata 

 in Bulacan on December 17. On December 19, 15 grams of arsenophenylglycin 

 were injected intravenously. Shortly after this injection the temperature which 

 had been elevated became normal. On December 27, 15 grams of arsenophenyl- 

 glycin were given intravenously. The blood remained negative until January 

 8, when trypanosomata reappeared. The animal was then inoculated with 5 

 grams of atoxyl, but did not bear the inoculation well and three days later 

 was in a very weak condition. On January 14 the blood examination showed 

 no parasites. On January 22, 24, and 26, 10 grams of arsenic sulphide were 

 given by mouth and on January 29, 5 grams of atoxyl subcutaneously. On 

 January 31, 20 grams of arsenic sulphide; February 2, 30 grams of arsenic 

 sulphide; February 5, 5 grams of atoxyl; February 7, 20 grams of arsenic sul- 

 phide; February 9, 20 grams of arsenic sulphide. February 14 the animal 

 became still weaker. He developed pressure sores, and on March 19 trypano- 

 somata reappeared in his blood and he was destroyed. 



Mule [American) No. 29. — This animal was also observed at Bulacan. It 

 appeared in excellent condition at the time the trypanosomata were found on 

 December 17, on which date 15 grams of arsenic sulphide were given by mouth. 

 Decembr 19 the blood was still positive for parasites and 5 grams of atoxyl were 

 injected subcutaneously. December 21, 20 grams of arsenic sulphide were given. 



