104 AKON AND HOCSON. 



cleaned his room and did other things. The experiments on this man 

 were divided into three periods, each covering four days. In the first, 

 a diet low in phosphorus was given, in the next, egg albumen, and in the 

 third, phytin was added. This man, weighing 64 kilos, received 400 

 grams of bread, 300 grams of rice, 75 grams of bacon and 100 grams 

 of sugar daily, thus giving him 41 calories per kilo of body weight. 

 More detailed information concerning the foodstuffs, etc., will be given 

 under the head of the next experiment, which was in part conducted 

 simultaneously with this one. Both men were naturally kept in two 

 different isolation rooms. 



A study of jDeriods V to VII shows that in the first period where the 

 man received bread, rice, bacon and sugar only, and took in daily 9.57 

 grams of nitrogen and 1.50 grams P 2 5 , the intake of nitrogen as well as 

 that of P 2 5 did not cover the needs of the body. The quantities 0.023 

 gram P 2 5 and 0.15 gram nitrogen per kilo of body weight are therefore 

 insufficient. The increase of nitrogen in the diet from 9.58 to 11.06 

 grams by the addition of egg albumen (period VI) reduced the loss of 

 nitrogen considerably, namely, from 4.67 to 1.98 grams per day. 



The loss of P 2 G at the same time is slightly decreased, as compared 

 with the foregoing period. While the first man, weighing 52 kilos, with 

 11 grams nitrogen per day was exactly in nitrogen equilibrium, the 

 other, of 64 kilos weight, with the same intake, lost nearly 2 grams of 

 nitrogen. The absolute requirement of both men would therefore amount 

 to approximately 0.2 gram of nitrogen per kilo of body weight. 



Finally, during the last period (VII), with the same intake of nitrogen 

 as in period V, but a large increase in that of P 2 , brought about by 

 the addition of 6 grams of phytin daily, 1.5 grams P 2 O were retained 

 daily from the amount given; this retention probably being caused by 

 the fact that more than 5 grams of P 2 5 had been lost from the body 

 during the two foregoing periods (V and VI). 



Special attention is called to the nitrogen metabolism. Whereas the 

 intake in period VII is about the same as in period V, the loss of 

 nitrogen from the body is not half so great. This influence of the 

 organic phosphorus upon the nitrogen metabolism is in accordance with 

 the observations of Mendel and TJnderhill, 21 Le Clerk and Cook, 34 

 Tunicliff 3S and others, mentioned above. It shows that nitrogen metab- 

 olism must not be forgotten while studying that of phosphorus under 

 different conditions of nutrition. 



The distribution of nitrogen and P 2 5 in the urine and fasces during 

 these experiments was as follows : The content of the f a?ces in nitrogen 

 is nearly the same during the three periods, being about 3.0 to 3.4 grams. 

 This amount would accord with nearly 35 per cent of the intake cor- 

 responding to the periods without the addition of protein (V and VII). 

 The values would agree fairly well with those found by other authors 



