PARTHENOGENESIS OP FEMALE CRESCENT BODY. 181 



recognizable as the stroma of the chromocyte. In addition, a slight 

 polychromatophilia was present; basophilia could not be observed. 

 However, there were peculiar, very large, retifonn, brick-colored cells 

 (macrophages from the spleen) and also a few macrocytes. 



After careful and repeated examination of the blood preparations, I 

 arrived at the conclusion that a mixed infection was certainly not 

 present, because only large rings of the tropical parasites, situated in 

 chromocytes showing Maurer's spots and crescent bodies, could be found. 



In addition to the blood constituents already described, the forms 

 shown in figs. 1, 2, and 3 were seen, in the slides. These were examined 

 with a Zeiss oil-immersion one-twelfth objective and No. i ocular, giving 

 a magnification of 950. However, as an exact representation of all the 

 details presented many difficulties when such an enlargement was used, 

 I sketched figs. 1, 2, and 3 on a scale about 1.5 times greater, and they 

 thus possibly represent an enlargement of 1,500 ; on the other hand, figs. 

 4 and 5 are magnified only 950 times. If, now, we examine figs. 1 

 and 2, it becomes evident that the parasitic body occupies almost all of 

 the red blood corpuscle, which is neither enlarged nor faded. In fig. 3 it 

 occupies approximately two-thirds of it; figs. 2 and 3 show some large 

 parasites and fig. 1 two which are very minute. An elongated oval form 

 may be seen in fig. 1. This is indented on the right margin and has an 

 obtuse and slightly deflected point at the right upper corner, this point 

 being quite similar to that of many young crescent bodies in the same 

 blood slide. On the other hand, the opposite pole of these young gametes 

 is either round or obtuse. In fig. 2 the parasite is oval, with the narrower 

 pole directed downwards, whereas that of fig. 3 resembles a crescent body 

 with a convex projection on the concave side. The protoplasm of the 

 malarial parasite is stained a very light violet-blue in figs. 1, 2, and 3 ; in- 

 figs. 1 and 2 blue predominates ; in fig. 3, violet-red, and this color of the 

 protoplasm corresponds exactly to that of the crescent bodies in the blood 

 slides. We find in this protoplasm in fig. 1, especially at the left-hand 

 upper corner parallel to the contour of the parasite, but not touching 

 the periphery, a distinct, band-like, light violet-red chromatic mass of 

 basic material in which may be observed small, dark red-brown, chromatic 

 bands which are generally situated at right angles to the former. In 

 these small bands may be seen one or two chromosomes (nuclear masses) 

 stained very dark purple. This light, red-violet basic substance is prob- 

 ably to be looked upon as a metamorphic change between the proper 

 chromatic material and the protoplasm of the parasite. Between this 

 broad, band-like basic mass and the periphery of the parasite we see 

 imbedded in fig. 1 small, detached, chromatic points. In fig. 2 the light 

 red-violet, band-like basic substance runs parallel to the periphery of the 

 parasite, but touches the circumference almost at every point and incloses 



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