TROPICAL BRONCHOMYCOSIS. 203 



5. The diagnosis of bronchooi'diosis can be made only by bacteriologic 

 methods. It is differentiated from phthisis by the absence of tubercle 

 bacilli and the negative animal inoculations; from bronchial spirochas- 

 tosis by the absence of spirochastaa ; and from endemic haemoptysis by the 

 absence of the ova of the trematode. 



6. Care should be taken before making the diagnosis of bronchomy- 

 cosis that the sputum is collected in sterile vessels and examined as soon 

 as possible, because sputa left exposed to the air frequently become con- 

 taminated in the Tropics with various species of nonpathogenic saccharo- 

 myces and oidia. Primary bronehoniycosis should be also differentiated 

 from those cases of chronic debilitating disease in which O'idium albicans 

 spreads from the mouth to the bronchi. 



OBSERVATIONS ON A NEW SPECIES OF EPIDERMOPHYTON FOUND IN 



TINEA CRURIS. 



In 1905 I separated dhobie itch from the ordinary forms of tinea 

 corporis and Macleod suggested for the affection the term tinea cruris. 

 I stated at the time that the eruption was caused by different species of 

 fungi, the commonest of which a few months later I termed Trichophyton 

 cruris. Pernet found and described a fungus from a case of tinea 

 cruris, and later I gave the name of Tr. perneti to this species. In 

 1907 Sabouraud made an investigation into an epidemic of tinea cruris 

 occurring in France and he also, came to the conclusion that tinea cruris, 

 or, as he prefers to call it, tinea inguinalis, should be separated from 

 tinea corporis. He created a new genus for the trychophy ton-like organ- 

 ism observed in the disease — Epidermophyton. The principal character- 

 istics of this genus are that the fungi do not attack the hair follicles, 

 do not produce suppuration, and that cultures show forms of degenera- 

 tion in a very short time. Sabouraud isolated only one species in all 

 of his cases; but in the Tropics, in my experience, there can not be any 

 doubt of the plurality of species of the fungi producing tinea cruris or 

 dhobie itch. The fungi described so far are: 



1. Epidermophyton cruris Castellani, 1905. E. inguinale (Sabouraud), 1907. 

 Colonies in maltose agar whitish, occasionally orbicular; later showing a 



greenish color. 



2. Epidermophyton perneti Castellani, 1907. Trychophyton perneti. 

 Colonies in maltose agar of a delicate pinkish color. The pinkish color is lost 



in subcultures. 



To these two species I am now in position to add a third, which I will 

 designate as E. rubrum and which I have isolated from two cases of the 



