TROPICAL BRONCHOMYCOSIS. 205 



The principal cultural characters of epidermophyton rubrum. 



Medium. 



Per cent. 



Characters. 



r 









2 





Mannite agar 



2 



Do. 





2 



Cream growth with white, powder-like surface. 

 Do. 



Maltose acid agar 



2 





2 



Do. 





2 



Do. 





2 



Do. 





2 



White growth with white, powder like surface. (After 

 1 month the growth on saccharose presented a white 











central knob surrounded by a grayish-white zone. 







This grayish- white zone was surrounded by a distinct 







yellow ring and outside this was another zone of 







gray white color.) 













White growth. Nonliquefied after 21 days. 











Alkaline. Complete separation in 7 days. 

 Growth on surface and at bottom of tube of pale yellow 

 color. 



Broth 









Peptone water- 





Growth at bottom of tube. White. 



Lactose-litmus broth 



2 



No acidity. Good surface growth of greenish-white 

 color. 



Mannite-litmus broth 



o 



No acidity. Good surface growth. White. 



Dulcite-litmus broth 



2 



No acidity. Good growth at bottom of tube. 



Glucose-litmus broth 



2 



No acidity. Good surface growth. Red with whitish 

 down. 



Maltose-litmus broth.. 



2 



No acidity. Superficial whitish growth. 



Saccharose-litmus broth 



2 



No acidity. Superficial greenish-white growth. 



Lfevulose-litmus broth __ 



2 



No acidity. Abundant greenish-white surface growth. 





2 



No acidity. Abundant greenish-yellow surface 

 growth. 







Ram nose-litmus broth 



2 



No acidity. Abundant greenish- white surface growth. 



Galactose-litmus broth-. 



2 



No acidity. Abundant greenish-yellow surface 

 growth. 



Dextrin-litmus broth _ . 



2 



No acidity. Only slight growth at bottom of tube, 

 none on surface. 



Arabinose-litmus broth 



2 



No acidity. Greenish-white surface growth. 



Adonite-litmus broth 



2 



No acidity. Growth only at bottom of tube and very 

 slight. 



Nutrose-litmus broth 



2 



No acidity. Growth at bottom of tube and also abun- 

 dant surface growth of greenish-yellow color. 



Observations on subcultures. — Subcultures from a Sabouraud or glucose culture 

 on Sabouraud or glucose media are deep red, like the original cultures, but 

 subcultures on agar, saccharine agar, and saccharose agar are white, greenish or 

 yellowish. Subcultures from an agar culture (white) or ordinary agar, saccharine, 

 and saccharose are white, but if Sabouraud agar tubes or glucose or mannite 



