TROPICAL BRONCHOMYCOSIS. 



Cultural characteristics of Hie spirillum. 



207 



Medium. 



Per cent. 



Characters. 











was distinctly i 







alkaline and peptonized.) 



Broth 





General turbiditv; pellicle. 



1 







General turbidity; slight pellicle 











Sprnm 





Do. 





Agar - 





Whitish. Coli-like. 





Lactose-litmus broth 



2 



No change. Fair growth. 





Saccharose-litmus broth 



2 



Do. 





Dulcite-litmus broth 



2 



Do. 





Mannite-litmus broth 



2 



Do. 





Glucose-litmus broth 



2 



Do. 





Maltose-litmus broth 



2 



Do. 





Dextrin-litmus broth 



1 



Do. 



I 



Raffinose-litmus broth 



1 



Do. 





Arabinose-litmus broth 



1 



Do. 





Adonite-litmus broth 



1 



Do. 







1 



1 



Do. 

 Do. 





Nutrose-Htmus broth 



Galactose-litmus broth 



1 



Do. 





Ltevulose-litnms broth _ . 



1 



Do. 





Indol 













Negative. Very motile. 









Pathogenicity. — The spirillum was pathogenic for guinea pigs and rabbits dur- 

 ing the first two weeks after its isolation, the animals dying in from twenty-four 

 to forty-eight hours after hypodermic injection of 2 cubic centimeters of a broth 

 culture or of 1 cubic centimeter intraperitoneally. After being isolated for a 

 longer time, the organism lost its pathogenicity. 



CONCLUSION. 



The cultural characteristics show the spirillum most probably to be a 

 new species, for which I propose the name of Spirillum zeylanicum. 



