CHOLERA AND CHOLERA-LIKE VIBRIOS. 405 



Table I. — Cholera and noncholera vibrios studied — Continued. 

 NONCHOLERA VIBRIOS STUDIED. 



No. 



Name. 



Date. 



Origin. 



Remarks. 



1 



2 

 3 

 4 

 5 



6 



7 



8 



9 

 10 

 11 

 12 

 13 

 14 

 15 

 16 

 17 

 18 



19 

 20 

 21 

 22 

 23 

 24 



No. 135 



1909. 

 Apr. 15 

 June ? — 

 June 6 

 Aug. 18 

 Sept. 8 



Sept. 11 



.do 



Human intestine- 



Amosbic dysentery. 



Estero at Calle Iris. 



Necropsy, pelvic peritonitis, salpingitis. 



Post-mortem decomposition of all organs. 



Mild case of cholera. This vibrio found 

 in addition to true cholera vibrios. 



Stagnant jjool which received human ex- 

 cretions. 



Specimen from water left in fishing boats 

 after the fish had been unloaded. 



From water in bottom of boats after fish 

 had been unloaded. 



From fishing boats. 



[Stagnant water which received house 

 | wastes, including human excrement. 



Large unprotected well without walls. 

 "Estero de Bilibid" which receives 

 human excrement. 





Notbil 



Human intestine. 

 do 







do ., . 



Water ' 



Sta. Monica 



do . 





do. 



do _ 





Oct.?_- 

 Oet, 14 

 do 



do 



Navotas A 



do - 





do 



do 













do _. 







..... do 







do 







. do 



-Bilibid 





do 



869 





Human intestine. 

 ' do„l 



568 





371 . 





do 



75- 





____ do_ 



48 





do .. 



21_ _ 





do 









Great difficulty was experienced formerly in securing bacteriological 

 diagnoses of suspicious cases of cholera occurring in distant provinces. 

 Often the examination of the specimens of fasces sent was futile. On 

 the other hand, it was found that if ordinary alkaline agar slants were 

 inoculated from the faeces and the cultures shipped in tin mailing cases 

 cholera vibrios could frequently be isolated from such tubes even after 

 they sometimes had been fifteen days in transit. 



Two points should be accentuated in giving directions to health officers 

 in distant places for the preparation of these cultures : (1) To place very 

 little material on the slant; (2) to secure the specimen in the first 

 twenty-four hours of the illness, if possible. When these directions are 

 followed, the tubes often present isolated colonies of cholera vibrios upon 

 arrival at the laboratory, and even if they do not appear so promising, 

 recovery of the vibrios from them is often not difficult by the ordinary 

 peptone and agar plate method. 



