CHOLERA AND CHOLERA-LIKE VIBRIOS. 



429 



Meinieke(5), Muhlens and Von Raven (7), Schumacker(ll), Neufeld and Haen- 

 del ( 8 ) , demonstrated that certain cholera strains possessed hsemolysing properties. 

 These workers used cultures which had been kept for a considerable time upon 

 laboratory media. Kraus(6), working with fresh cholera cultures, was unable to 

 find a strain with hsemolysing properties. 



The hemolytic action of our vibrios was tested by the simple method employed 

 by Ruffer and also by the method of Kraus. One loop of an eighteen-hour culture 

 was suspended in 5 cubic centimeters of normal salt solution. To 0.5 cubic cen- 

 timeter of this suspension, 0.5 cubic centimeter of a 5 per cent suspension of well- 

 washed blood corpuscles was added. The mixture was placed in the thermostat 

 at 37° and results noted after two hours, four hours, and twenty-four hours. This 

 method was also used by Neufeld and Haendel. By this means Ruffer found no 

 strain of cholera with hemolytic properties, while Neufeld and Hosdel found that 

 several cholera strains were capable of producing haemolysis. However, none of 

 these were freshly isolated. 



Our cholera strains, both fresh and old. uniformly failed to produce 

 haemolysis with these methods. Both El Tor strains examined were 

 hemolytic, as well as 18 of the 22 noneholera vibrios. The results are 

 shown by Table III. 



Table III. — Hcemolytie tests. 

 NONCHOLERA VIBRIOS. 



Name of culture. 



Ruffer's method, suspension 

 of live vibrios. 



Kraus's method, 0. 1 cc. 3-day 

 bouillon, killed to 1 cc. 5 per 

 cent suspension red cells. 



2 hours. 



4 hours. 



24 hours. 



2 hours. 4 hours. 



24 hours. 





+ 

 + 



+ + 



+ 



+ + + 



+ + 



+ 4- 



4- + + 

 + + + 

 + + + 





+ 4- 



+ + 



+ -1 

 4- + + 



+ + 

 + + + 

 4- 4- 4- 



+ 



H 



H 



+ 

 4- 4- + 



+ 

 -1- H 

 + + 

 + + 



+ + 



+ + 



+ 



Notbil 











+ 

 + 

 + 



+ 

 + 

 + 



+ + 





+ 

 + + 



tr. 

 tr. 

 tr. 

 tr. 





+ + + 



4-4-4- 













Carolina. 



135_ 



+ + 4-4-4- 













+ + 

 4- 4- 



+ + + 

 4-4-4- 

















+ + + 

 4- 



. , , 



tr. 



4- 4- 



Bilibid .. 



4- 4- 



— tr. 



869 . 



4- 4-4-4- 





+ 

 + 

 4- 



+ 

 + 



846 



+ 



+ + 

 + 



+ 4-4- 



+ + + 



+ 4- 4- 

 + + + 

 + + + 





568 _ 





371. 





- 



75 - 



48 



4- -J- + 





21 





+ 











