458 DUDLEY AND WHITMORE. 



of these rabbits. This strain was carried through three passages in 

 rabbits between May 31 and July IS, all of the last passage rabbits dying 

 of typical rabies in about two weeks. It was not considered necessary 

 to carry this strain any further, so no more inoculations were made, it 

 being preserved in neutral glycerine in the refrigerator. 



We had now shown so conclusively that rabies does occur in dogs in the 

 Philippine Islands and in the human in Manila that it was necessary for 

 us to secure a strain of fixed virus and carry it along in Manila. It did 

 not seem advisable to wait for one of our own strains to become '''fixed," 

 as a year or more probably would be required to reach that end ; and four 

 attempts to bring in a fixed virus had already failed, the virus in each 

 instance losing its virulence en route or else very soon after its arrival. 

 Accordingly, one of us (Whitmore) was sent to Saigon, French Indo- 

 China, to study the methods of the Pasteur Institute for preventive 

 treatment of rabies and to bring back if possible a fixed virus in animals 

 and in glycerine. The fixed virus in use at Saigon was brought out from 

 the Paris Institute some months ago. It had gone through upward of 

 eight hundred passages in Paris and fifteen in Saigon. 



Babbits were inoculated subdurally with fixed virus at the Saigon 

 Institute and were placed on a boat sailing directly for Manila. At the 

 same time fixed virus was placed in a bottle of neutral glycerine in a 

 refrigerator on the same boat. The rabbits arrived in Manila in good 

 condition and died of experimental rabies in the Biological Laboratory, 

 Bureau of Science. 



The fixed virus in glycerine had preserved its full virulence and when 

 inoculated subdurally into rabbits in Manila killed them in about ten 

 days. The brain tissue from the imported rabbits was also fully virulent 

 when inoculated into rabbits here. This strain of fixed virus has now 

 passed through five passages of rabbits in the Biological Laboratory, 

 Bureau of Science, and constantly kills rabbits in about ten days, with 

 typical symptoms of experimental rabies. This strain is being used for 

 the protective inoculation against rabies which is now being carried on 

 in Manila. 



SUMMARY. 



1. We have shown conclusively that rabies occurs among dogs in the 

 Philippines, (a) by finding Negri bodies in the brain tissue of two clogs 

 killed for suspected rabies; (b) by carrying the virus from the brains 

 of one of these clogs through seven passages in rabbits, rabbits of the 

 seventh passage dying in nine days of typical experimental rabies ; 

 (c) by demonstrating the presence of Negri bodies and Lentz passage 

 bodies in the brain tissue of these rabbits up to the third passage. 



2. We have shown conclusively that rabies does occur in the human 

 in Manila, (a) by observing a case that was clinically typical rabies; 



