550, SHAKLEE. 



4.30: Lying quietly; eyes closed; breathing 24 per minute, quiet, regular, 

 practically normal. Pulse 60, full, strong, slightly irregular. Starts when dis- 

 turbed. Refused drink. 



On the next day the dog seemed in full possession of its faculties, but some- 

 what stupid or listless. On the third day the dog ate and drank, but was 

 still stupid. Later on it began to decline and on the seventh day died. The 

 gross appearance of the organs after death was not strikingly abnormal, but the 

 lungs, heart and kidney seemed congested. A microscopic study of the organs 

 will be made later. 



Protocol No. 8. — Dog, 9.1 kilos, g , Brown native, lean, probably 5 years old, 

 mangy. 



1.25: 1 cubic centimeter morphine sulphate (4 per cent) subeutaneously. 



2.00: 0.5 cubic centimeter atropine sulphate (1 percent) intravenously. 



2.05: Chloroform 2/6 with insufflation. 



2.11: Chloroform 5/12, pulse 140 soft, marked venous pulse in neck, respiration 

 40, moderately deep, slightly irregular. 



2.17: Pulse 156, respiration 48, shallow, irregular. 



2.10: 0.73 cubic centimeter strychnine sulphate (1 per cent) injected with 

 Ringer-Locke solution, the injection of the latter solution was continued. 



2.20: Strong tetanus, chloroform 3/6 with insufflation. 



2.21: Eyes open and pupils wide. No convulsions, but limbs held in extension. 

 Respiration shallow. 



2.23 : No lid reflex. Slight rythmic movement of hind legs. 



2.31 : Chloroform 5/12 with insufflation. No lid reflex. Respiration 42, deep, 

 fairly regular. 



2.37: Lid reflex fairly good. Chloroform 3/6 with insufflation. 



2.39.5: No lid reflex, chloroform 5/12 with insufflation. 



2.44: Slight lid reflex, foreleg still in extension and hind leg still in rythmic 

 motion. 



2.55: Chloroform 2/6 with insufflation. 



2.59 : Lid reflex good, respiration good, rythmic movements of fore legs, pulse 

 168, small, moderately strong, regular. 



3.13: Fore leg relaxing. 



3.24: Hypersensitive, lid reflex sharp, heart and respiration in good condition. 



3.39: Sudden start, chloroform increased to nearly 5/12 with insufflation. 



3.44: Injection of Ringer-Locke solution stopped, 900 cubic centimeters in all. 



3.55: Chloroform 2/6. 



4.01 : Chloroform and insufflation off. 



5.00 : The dog has gradually recovered from the chloroform and is now attempt- 

 ing to rise to its feet. 



On the following day the dog seemed normal, bright and strong;" but the third 

 day at 8.15 a. m. he began to droop and at 9 a. m. died quietly, about forty-eight 

 hours after the beginning of the experiment. At autopsy the heart and liver were 

 found very pale and yellow, suggesting marked fatty degeneration. The same 

 appearances were found at other autopsies. 



Results. — Of the first ten dogs of this series, one was given a small 

 dose of eurarine followed later by ether. This was the only one of the 

 series that made a permanent recovery, in spite of the fact that the 

 treatment was carried out by third-year medical students without 

 previous experience in treatment of strychnine poisoning. The remain- 

 ing nine dogs were treated with chloroform. Some had morphine as 



