560 WHITMORE AND CLEGG. 



site of the injection, and finally a small abscess would form and dis- 

 charge a sero-purulent material. 



Eleven cases of leprosy were treated with this vaccine for eight 

 months, and twenty-one for seven months. At the end of the treatment 

 there was no evidence of improvement in the condition of any of ■ the 

 cases, and, as we observed the formation of abscesses as soon as we 

 increased the dose, it was decided to change to some other preparation. 



We next employed a glycerine extract made from our acid-fast bacillus 

 in the same way that old tuberculin is obtained from the tubercle bacillus. 

 We tried the extract on leprous, tuberculous, and normal persons in 

 the manner of the von Pirquet skin test for tuberculosis. Not one 

 of the persons showed any reaction to the material. 



We gave all the cases of leprosy in our vaccine series injections of 

 this extract once a week, increasing the dose until 10 milligrams were 

 administered at a dose at the end of two months. 1ST one of the patients 

 had improved and no reactions had followed the injections. 



Our next preparation was an emulsion of our acid-fast bacillus in 

 a 1 to 60 aqueous solution of sodium oleate. The cultures on glycerine 

 agar were scraped off and emulsified in the soap solution, so that one 

 cubic centimeter of the solution would contain one milligram of moist 

 bacteria. This emulsion was placed in the shaking machine for three 

 days and then heated to 60° for one hour. The bacteria were nearly 

 dissolved, there being only a slight sediment on allowing the solution 

 to stand. When the preparation was shaken, the sediment was dis- 

 tributed freely through the solution without any tendency to form 

 clumps. This preparation was sterile and remained so without the 

 addition of any other preservative. 



All the leprosy cases in our vaccine series were given injections of 

 this new vaccine once a week. The dose was gradually increased until 

 the patients were receiving one cubic centimeter of the emulsion, rep- 

 resenting one milligram of moist bacterial substance, at a dose. Two 

 cases developed a sharp reaction, with fever and malaise. There was 

 considerable swelling and redness around the leprous lesions, especially 

 on the face, hands, and feet. In no case was there local reaction at 

 the site of injection, and no abscesses were observed. At the end of 

 two and one-half months there was no improvement in the condition of 

 any of the patients. 



We next employed the spleens of patients dead of leprosy. We chose 

 a spleen that was shown microscopically to be very rich in leprosy 

 bacilli. We ground up this tissue and added a 1 to 60 aqueous solution 

 of sodium oleate, so that one cubic centimeter of the resulting mixture 

 would represent 0.5 gram of spleen substance. This mixture was shaken 

 in the shaking machine for three days, filtered through cotton, and heated 

 to 60° C for one hour. On standing, a small amount of sediment col- 



