SPECIFIC TREATMENT OF LEPROSY. 561 



lected at the bottom, leaving the supernatant fluid clear and yellowish in 

 color. This clear fluid was used for the injections. It was sterile and 

 remained so without the addition of any other preservative. 



All of the leprosy cases in our vaccine series were given injections 

 of this preparation once a week. The dose was gradually increased 

 until the patients were being given one cubic centimeter of the solu- 

 tion, representing 0.5 gram of leprous spleen tissue, at each injection. 

 The cases have been receiving injections of this preparation for two 

 and one-half months. None of them show any improvement in their 

 condition and none of them have had any reaction after the injections. 



When we started our vaccine work we put three cases of leprosy on 

 weekly injections of atoxyl, and three on weekly injections of a mixture 

 of sodium cinnamate and mercuric cinnamate. Injections were con- 

 tinued in these cases for eight months. None of the patients improved 

 and none had reactions after the injections. We put our three "cin- 

 namate" cases on injections of sodium cinnamate and a glycerine extract 

 of our acid-fast bacillus, but at the end of three months there was 

 no improvement and we discontinued the method of treatment. 



Deycke injected cinnamic acid in order to produce a leucocytosis, and 

 observed a greater reaction from Nastin in conjunction with cinnamic 

 acid than from Nastin alone, but otherwise there was no result following 

 the cinnamic acid injections: 



At the time we began the specific treatment of our series of cases 

 of leprosy, for nine months Doctor Teague 3 of this laboratory had been 

 treating six cases of leprosy with injections of Nastin. He turned these 

 patients over to us and we further treated them with the same injec- 

 tions for eight months. Every seven days each individual was given 

 an injection of one cubic centimeter of Nastin B. At the end of eight 

 months- there was no improvement in the condition of any of the patients 

 and none had any reaction after the injections. 



SUMMARY. 



1. We have prepared a vaccine, a glycerine extract and a soap solution, 

 from an acid-fast bacillus which Clegg cultivated from leprous tissue. 

 We have used these preparations in the treatment of cases of leprosy for 

 twelve and one-half months, without noting any improvement in the con- 

 dition of any of the patients. In some of the cases we have noted reac- 

 tions after the injections, but we are not prepared to say whether or 

 not these reactions are specific. 



2. Our glycerine extract of this organism does not produce a skin 

 reaction on leprous or tuberculous patients, nor on normal persons. 



3 This Journal, Sec. B (1909), 4, 329. 



