﻿38 MUSGRAVE. 



A. MATERIAL. 



The character and the amount of material for this report has already 

 been extensively reviewed in Chapter V. 



B. METHODS. 



Museum specimens have been prepared by the Kaiserling method and 

 a fairly complete collection of specimens deposited in the Museum of the 

 Biological Laboratory of the Bureau of Science in Manila. Another 

 smaller selection has been forwarded to the Army Medical Museum in 

 Washington, and still others furnished the Xaval School of Tropical 

 Medicine in Washington and other interested persons. Tissues for histo- 

 logic study were fixed in Zenker's solution and in alcohol and ultimately 

 imbedded in paraffin. 



A large variety of stains has been used, including Van Gieson's, 

 Heidenhain's iron and other hematoxylins, Borrel's and the safranin 

 stains. Manj' stains are satisfactory for the reason that fine differentia- 

 tion is hardly necessary for the study in sections of the organisms or eggs. 

 On the whole, the Van Gieson has proved the most useful stain, and it 

 is by far the most satisfactory for the study of the cuticle and spines 

 of the parasites. Eosin and methylene-blue give a sharp and clear 

 picture, particularly of the eggs, although these, as well as the parasites, 

 are easily recognized even in unstained sections. 



