﻿EXPERIMENTAL INVESTIGATIONS REGARDING THE ETIOL- 

 OGY OF DENGUE FEVER, WITH A GENERAL 

 CONSIDERATION OF THE DISEASE. 1 



By P. M. Ashbubn and Charles F. Craig. 2 



(From the Laboratory of the United States Army Board for the Study of Tropical 



Diseases, Division Hospital, Manila, P. I., and the Biological 



Laboratory of the Bureau of Science.) 



CONTENTS. 



I. Introduction. 

 II. Epidemiology. 



1. Historical. 



2. The Fort William McKinley epidemic. 



III. Etiology. 



1. Historical. 



2. Examination of the blood. 



(a) Haemoglobin. 



( b ) Erythrocytes : Number, morphology and staining reac- 



tions. 



(c) Leucocytes: Number, morphology, varieties and differential 



counts. 



(d) Blood plates. 



(e) The blood plasma. 



3. Blood culture in dengue. 



(a) Methods. 



( 6 ) Citrated blood culture. 



(c) Bouillon blood culture. 



4. Intravenous inoculation of unfiltered dengue blood. 



5. Intravenous inoculation of filtered dengue blood. 



6. Experimental transmission of dengue by the mosquito. 



7. Experimental period of incubation in dengue. 



8. Immunity and susceptibility. 



9. Contagion in dengue. 



IV. Symptomatology. 

 V. Diagnosis. 



VI. Treatment. 

 VII. Conclusion. 



1 Eead by abstract at the Fourth Annual Meeting of the Philippine Islands 

 Medical Association, March 1, 1907. 



2 P. M. Ashburn, captain and assistant surgeon, United States Army, and 

 Charles F. Craig, first lieutenant and assistant surgeon, United States Army, 

 constituting the United States Army Board for the Study of Tropical Diseases as 

 they Occur in the Philippine Islands. 



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