﻿VI. SEEIES OP ANIMAL INOCULATIONS EMPLOYED IN 



TESTING THE IMMUNIZING VALUE OP THE 



DIFFERENT METHODS. 



EXPERIMENTS DEMONSTRATING SUSCEPTIBILITY OF MONKEYS 

 TO PLAGUE INFECTION. 



Series 1. — With monkeys. 



One 48-hour agar slant culture of "Pest Virulent" was suspended in 5 cubic 

 centimeters of bouillon. A 10 cubic centimeter syringe needle was dipped in such 

 a suspension and the needle then thrust beneath the shaved skin of each animal 

 in the region near the root of the tail, pushed in full length and then withdrawn. 



Animal 

 No. 



Result. 



Autopsy and remarks. 



1222 



1223 



2695 

 2696 



Dead in 3 days __. 



Dead in 4 days 



.do _ 



Pest septicaemia. B. pestis from heart, liver and spleen and hemor- 

 rhagic inguinal glands. 

 Pest septicemia. Innumerable pest bacilli in blood of heart. 

 Pest septicaemia. B. pestis from heart, spleen and liver. 

 Pest septicaemia. B. pestis from heart, spleen and liver. 



Dead in 3* days _. 



Series 2. — With monkeys. 



One 48-hour agar slant culture of "Pest Virulent" (second transplant from 

 guinea pig number 1220) suspended in 5 cubic centimeters of bouillon. One 

 cubic centimeter of the suspension equal to 2 oesen. Animals both inoculated 

 subcutaneously near root of tail with 1 cubic centimeter. 



Animal 

 No. 



Amount 

 inoculated. 



Result. 



Autopsy and remarks. 



1224 

 1225 



1 cc. (=2 oesen) 

 subcutaneously. 



.do 



Dead in 3 



days. 



Dead in 2 

 days. 



Pest septicaemia. Smears from the heart, spleen, 

 haemorrhagic glands and liver show numerous pest 

 bacilli. 



Pest septicaemia. Haemorrhagic area about point of 

 inoculation. Left inguinal glands swollen and 

 haemorrhagic. Spleen swollen. Innumerable pest 

 bacilli from all organs. 





55670- 



187 



