﻿188 



STRONG. 



Series 3. — With monkeys and guinea pi'/*. 



One 48-hour agar slant culture of "Pest Virulent" suspended in 5 cubic centi- 

 meters of bouillon. A scalpel was dipped several times in such a suspension 

 and then rubbed over a shaved area on the abdomen, which was also slightly 

 scarified with the knife. 



Animal 

 No. 



Result. 



Autopsy and remarks. 



Monkey 

 1226. 



Monkey 

 1227. 



Monkey 

 1286. 



Monkey 

 1287. 



Dead in 5 

 days. 



Dead in 4 



days. 

 Alive. _ - 



do____ 



Found dead in the morning. On microscopical examination of 

 stained-blood films from heart's blood, pest bacilli found present in 

 very small numbers. Cultures from the heart developed colonies 

 of the pest bacillus. A portion of the spleen of this animal was rubbed 

 over the shaved abdomen of monkey No. 1244 (see below). 



Plague septicaemia. Hemorrhagic glands. Cover slip specimens from 

 heart's blood reveal innumerable pest bacilli. 



The abdomen of each animal was shaved over a small area and scarified lightly, 

 then a portion of the spleen of another animal which had succumbed to acute 

 pest infection was rubbed over this area. 



Animal No. 



Abdomen massaged 

 with spleen of — 



Result. 



Autopsy and remarks. 



Monkey 1244_ 



Guinea pig 

 1170. 



Guinea pig 

 1296. 



Monkey No. 1226 



(see above). 

 Monkey No. 1288 



(see Series 5, Oct. 



25). 



_ do 



Alive and well. 



Dead Oct. 30, 

 after 5 days. 



__ .do 



Buboes and multiple necrotic foci in 

 spleen and liver. Pure cultures of 

 Bacillus pcstis from the heart. 



Buboes and multiple necrotic foci in 

 spleen and liver. Numerous pest ba- 

 cilli in smears from spleen. 







Series 16. — With monkeys. 



On November 2, two 48-hour agar slant cultures of "'Pest Virulent" (from 

 guinea pig number 1296, second transplant) were suspended in 10 cubic centi- 

 meters of bouillon (5 cubic centimeters to each tube), a 10 cubic centimeter 

 syringe needle was dipped in this suspension and thrust beneath the skin of 

 monkeys numbers 1357 to 1360 inclusive. Monkeys numbers 1361 to 1364 in- 



