﻿STUDIES IN PLAGUE IMMUNITY. 



Series 1G — Continued. 



189 



elusive each received subcutaneously 0.5 cubic centimeter of this same suspension 

 (equal to 1 oese "Pest Virulent") and monkeys numbered 13G5 to 1368 each 

 received subcutaneously 0.25 cubic centimeters, equal to ^ oese. 



Animal 

 No. 



How inoculated. 



Result. 



Remarks and autopsy. 



1357 



1358 

 1359 



1360 



1361 



1362 



1363 

 1364 

 1365 



1366 



1367 

 1368 



Stuck with 10 cc. 

 needle dipped in 

 suspension "Pest 

 Virulent." 

 .do 



Alive and well _ 

 .do 



Animal suffered a mild infection and became 

 immune, resisting the reinoculation of 2 whole 

 oesen "Pest Virulent" on Dec. 14. 



Do. 

 Innumerable pest bacilli in smears from the 



spleen. 

 Innumerable pest bacilli in smears from the 



spleen. Pest septicsemia. 

 Innumerable pest bacilli in smears from the 



spleen. 



Do. 



Do. 

 Do. 

 Do. 



Do. 

 Do. 

 Do. 



do 



Dead Nov. 7, 

 after 5 days. 



Dead Nov. 9, 

 after 7 days. 



Dead Nov. 6, 

 after 4 days. 



Dead Nov. 5, 



after 3 days. 

 do 



do 



1 oese "Pest Viru- 

 lent" subcutane- 

 ously. 



do 



do 



do 



do 



i oese "Pest Viru- 

 lent" subcutane- 

 ously. 

 _ _ do__ ___ .... 



Dead Nov. 7, 

 after 5 days. 



do 



do 



____ do __ . 



___do 



Dead Nov. 6, 

 after 4 days. 





EXPERIMENTS IN IMMUNIZATION OE ANIMALS WITH KILLED 

 CULTURES OE THE PEST BACILLUS. 



Series 5. — Killed bouillon cultures, with monkeys. 



The following monkeys were inoculated subcutaneously near the root of the 

 tail with Haffkine's plague prophylactic purchased by the Bureau of Health from 

 India. The immunity of all the animals was tested eleven days after the first 

 injection, in the following manner: One 24-hour agar slant culture of "Pest 

 Virulent" was suspended in 5 cubic centimeters of saline solution; a 10 cubic 

 centimeter syringe needle was dipped in this suspension and then thrust beneath 

 the shaved skin of the animal on the opposite side of the tail to which the first 

 injection had been made. 



