﻿190 



STRONG. 



Series 5 — Continued. 



Animal 

 No. 



Amount of 

 prophylac- 

 tic inocu- 

 lated. 



Result after 

 infection. 



Autopsy and remarks. 



1234 



5 ce - _ 



Dead after 5 days_ 



Pest septicaemia. Innumerable pest bacilli in heart's 

 blood. 



1235 

 1236 



5cc 



do 



Do. 

 Numerous pest bacilli in smears from spleen. 

 Extensive haemorrhages in the mesentery. Numerous 



10 cc 



do 



1237 



30 cc - 



Dead after 7 days. 









pest bacilli in smears from spleen. 



1238 



20CC _ - 



Dead after 14 days. 



Only one or two bacilli found in smears from spleen. 









Innumerable in smears from large necrotic bubo near 

 region of left buttock (point of inoculation) — chronic 

 pest. 



1239 

 1240 

 1241 

 1288 



20cc. _ 

 10 cc _ 



Alive. 



Numerous pest bacilli in smears from the blood and 



... do _ _ 



30 cc 



..do . 



Control 



Dead after 3* days_ 



■ 







spleen. 



1289 



Control _._ 



Dead after 7 days. 



Numerous pest bacilli in smears from spleen. 



Series 9. — Killed agar cultures "Pest Virulent," with monkeys. 



Six 48-hour agar slant cultures of "Pest Virulent" ( from guinea pig number 

 1221. second transplant) suspended in 6 cubic centimeters of saline solution (1 

 cubic centimeter to each tube), the whole mixed and heated for one and one-half 

 hours at 62° C. A culture taken proved the suspension to be sterile. The follow- 

 ing animals were inoculated subcutaneously, each with 2 cubic centimeters (equal 

 to 2 agar slant cultures) on October 18. On October 28 the animals were reinoc- 

 ulated subcutaneously on the other side of the body with 1 cubic centimeter of a 

 suspension of a 48-hour slant culture of living "Pest Virulent" in 5 cubic cen- 

 timeters of bouillon (1 cubic centimeter equal to 2 oesen). 



Animal 

 No. 



Immunized 

 October 18. 



Infected October 28. 



Result. 



Autopsy and remarks. 



1261 

 1262 

 1263 



2 agar slant 

 cultures, 

 do 



2 oesen "Pest Virulent" 



subcutaneously. 

 do 



Dead after 

 4 days. 



Dead after 



3 days. 

 do _ 



Pest septicaemia. 



Numerous pest bacilli in 

 smears from spleen. 



Innumerable pest bacilli in 

 smears from spleen. 



do 



do " 









CONTROL. 



1322 L _ _ _ _ 



2 oesen "Pest Virulent" 

 subcutaneously. 



Dead after 

 3 days. 



Innumerable pest bacilli in 

 spleen. 



