﻿STUDIES IN PLAGUE IMMUNITY. 

 Series 25 — Continued. 



193 



inoculated subcutaneotisly each with 1 cubic centimeter, monkeys numbered 1572 

 to 1576 each with 2 cubic centimeters and number 1577 with 0.8 cubic centimeter. 

 Three of the animals apparently succumbed from the effect of this inoculation. 

 On December 26, eighteen days after the first inoculation, the animals were 

 reinoculated in the following manner: Six 48-hour agar slant cultures of "Pest 

 Virulent" (from guinea pig number 1592, second transplant) were suspended in 

 30 cubic centimeters bouillon (5 cubic centimeters to each tube). Each animal 

 was inoculated subcutaneously on the opposite side of the body to that on which 

 the first inoculation was made with 0.33 cubic centimeter, equal to § oese "Pest 

 Virulent." Twelve control animals were also inoculated at the same time with 

 the same amount of the suspension. 



Animal 

 No. 



Inoculated Decem- 

 ber 8 subcutane- 

 ously with — 



Reinoculated 



December 26 



with — 



Result. 



Autopsy and remarks. 



1558 



1 killed culture 



§ oese "Pest 



Dead Dec. 28, 



Innumerable pest bacilli in 





"Pest Virulent." 



Virulent." 



after 2 days. 



smears from spleen. 



1559 



.do 



do 



Dead Jan. 10, 



Chronic pest. Very numerous 









after 15 days. 



pest bacilli in smears from 



1560 



do 



do 



Alive. 



spleen. 



1561 



do — _ 



do 



Dead Dec. 28, 

 after 2 days. 



Fair number of pest bacilli in 

 smears from the spleen. Very 















numerous about point of 











inoculation. Tissues cedema- 











tous and haemorrhagic. 



1562 



do_- 



do 



Dead Dec, 28, 

 after 2£ days. 



Numerous pest bacilli in smears 

 from the bubo. Culture from 















spleen negative. 



1563 



.... do 



do 



Dend Jan. 5, 

 after 10 days. 



Numerous pest bacilli in smears 

 from spleen. Large bubo. 







1564 

 1565 



do 



do 



Alive and well- 

 Died of first 

 inoculation. 



Nothing to suggest pest about 

 point of inoculation or in the 



___ do 

















spleen. Smears from spleen 











negative. 



1566 



do 



§ oese "Pest 

 Virulent." 



Dead Jan. 4, 

 after 9 days. 



Numerous pest bacilli in smears 

 from spleen. Bubo. 





1567 



.. .do 



....do 



Dead Dec. 30, 

 after 4 days. 



Few organisms in smears from 

 the spleen. Innumerable 















about point of inoculation. 











Intense hemorrhagic reaction. 



1568 



do 



do 



Dead Dec. 27, 

 after li days. 



Innumerable pest bacilli from 

 point of inoculation. 







1569 



do 



do 



Dead Jan. 2, 

 after 7 days. 



Few bacilli in smears from 

 spleen. Innumerable from 















bubo. 



1570 



do 





Dead Dec. 11, 



from primary 



Nothing to suggest pest except 

 small haemorrhages in the 













inoculation. 



lungs. Nothing suggestive at 

 the point of inoculation. Cul- 

 tures from the spleen negative 

 although large amount is 

 inoculated. 



1571 



do— 



J oese "Pest 

 Virulent." 



Alive and well- 







