﻿STUDIES IN PLAGUE IMMUNITY. 



195 



Series 48. — Killed agar cultures of "Pest Virulent" with monkeys. 



Fifteen 48-hour agar cultures of the strain "Pest Virulent" from guinea pig 

 number 2583, second transplant, were suspended in 15 cubic centimeters of saline 

 solution (1 cubic centimeter to each culture), the entire suspension heated for 

 one hour and fifteen minutes at 65° C. Cultures taken from the suspension 

 remained sterile. 



On October 27 each monkey of the series was inoculated subcutaneously with 

 1 cubic centimeter of the suspension (equal to one agar slant culture). On 

 November 28, one month after the inoculations, the immunity of the animals 

 was tested by the subcutaneous injection of 0.33 cubic centimeter of a suspension 

 of the strain "Pest Virulent" (five 48-hour agar cultures from guinea pig 

 number 2865, second transplant in 25 cubic centimeters bouillon) into the opposite 

 side of the body to that on which the vaccination was made. Twenty-five control 

 animals were also inoculated on this date and in the same manner. The immunity 

 of the animals of this series was tested on the same date and in the same manner 

 as was that of the animals comprising Series 40, 51, and 52 (pp. 197, 203, 217) . 



Animal 

 No. 



2664 



2665 

 2666 

 2667 



2668 



2669 



2670 



2671 



2672 



2673 



2674 



2675 



2676 

 2677 



2678 



Vaccinated October 



27 subcutaneously 



with — 



One killed 48-hour 

 culture "Pest Vir- 

 ulent." 



do 



do 



do 



_do. 



-do. 

 _do. 



-do. 



-do. 



-do. 



-do. 



.do. 



-do. 

 _do. 

 .do. 



Reinoculated 



November 28 



with — 



oese "Pest 

 Virulent." 



-do. 

 _do. 

 .do. 



-do. 



_do. 



Not reinocu- 

 lated. 



§ oese "Pest 



Virulent." 

 do 



Ido. 



_do. 



_do. 



_do. 

 -do. 

 _do. 



Result. 



Dead Dec. 2, 



after 4 days. 



Alive and well. 



do 



Dead Dec. 1, 



after 3 days. 

 do 



Dead Dec. 2, 

 after 4 days. 



Dead Nov. 5, of 

 first inocula- 

 tion. 



Dead Dec. 3, 

 after 5 days. 



Dead Dec. 1, 

 after 3 days. 



Dead Dec. 3, 

 after 5 days 



Dead Dec. 1, 

 after 3 days. 



Dead Dec. 2, 

 after 4 days. 



Alive and well. 



do 



Dead Dec. 1, 

 after 3 days. 



Autopsy and remarks. 



Few pest bacilli in smears from 

 spleen. Numerous at point of 

 inoculation. Buboes. 



Innumerable pest bacilli in 



smears from spleen. 

 Innumerable pest bacilli in 



smears from spleen. 

 Few pest bacilli in smears from 



spleen. 

 Careful post-mortem does not 



disclose cause of death. No 



marked local reaction. 

 Buboes and pest bacilli. 



Do. 



Do. 



Do. 



Innumerable pest bacilli in 

 smears from spleen. 



Fair number of pest bacilli in 

 smears from spleen. 



