﻿STUDIES IN PLAGUE IMMUNITY. 



197 



Series 49. — Killed bouillon cultures of "Pest Virulent," with monkeys. 



The monkeys of this series were inoculated on October 27, subcutaneously near 

 the root of the tail with from 10 to 15 cubic centimeters of six weeks' old 

 bouillon cultures of the virulent pest organism killed by heating for one hour 

 at G5° C. On November 28, one month after the primary inoculation, the 

 immunity of the animals was tested by the subcutaneous injection of § oese of 

 the strain "Pest Virulent" (from a 48-hour agar culture from guinea pig number 

 2865, second transplant), suspended in 0.33 cubic centimeter saline solution. 

 The animals were inoculated on the opposite side of the body from that upon 

 which the primary inoculation was made. Twenty-five control animals, numbered 

 2896 to 2920, were also inoculated on this date and in the same manner. They 

 all died of pest infection. For the details of the autopsies see Series 48. The 

 immunity of the animals of this series was tested on the same date and in the 

 same manner as was that of the animals comprising Series 48, 51 and 52, pp. 195, 

 203, 217. 



Animal 

 No. 



Inoculated October 



27 subcutaneously 



with — 



Reinoculated 



November 28 



with — 



Result. 



Autopsy and remarks. 



2697 



2698 



2699 

 2700 



2701 



2702 



2703 

 2704 



2705 



15 cc. killed bouil- 

 lon culture. 

 do . . 



§ oese "Pest 



Virulent." 

 do . 



Dead Dec. 7, 



after 9 days. 

 Dead Dec. 5, 



after 7 days. 

 Alive and well _ 

 Dead Dec. 5, 



after 7 days. 

 Dead Dec. 8, 



after 10 days. 

 Dead Dec. 3, 



after 5 days. 

 Alive and well .. 

 Dead Dec. 1, 



after 3 days. 

 Dead Dec. 2, 



after 4 days. 



Bubo containing pest bacilli. 

 Buboes containing pest bacilli. 



Do. 

 Do. 

 Do. 



Do. 

 Do. 



__ _do . . 



do 



do 



. .do 



.. do 



do 



10 cc. killed bouil- 

 lon culture. 

 . do 



do 



do 



. do .. 



do . 



do 



do 







Sehies 50. — Killed agar culture of "Pest Virulent," with guinea pigs. 



Fifteen 48-hour agar cultures of the strain "Pest Virulent" from guinea pig 

 number 2583, second transplant, were suspended in 15 cubic centimeters of saline 

 solution (1 cubic centimeter to each culture). The entire suspension was heated 

 for one hour and fifteen minutes at 65° C. Cultures taken from the suspension 

 remained sterile. On October 27 each guinea pig of the series was inoculated 



