﻿198 



STRONG. 



Series 50 — Continued. 



subeutaneously with 1 cubic centimeter of the suspension, equal to 1 agar slant 

 culture. On November 26, one month after vaccination, the immunity of the 

 animals in each instance was tested with the strain "Pest Virulent" in exactly 

 the same manner as in Series 46. 47, and 54 (pp. 211, 220, 232). Twenty-five 

 control animals, numbered 2870 to 2894, were inoculated at the same time and 

 in the same manner, all of which died of acute plague infection. For the details 

 of the protocols see Series 46 (p. 211). 



Animal 

 No. 



2(179 



2680 

 2681 

 2682 



2683 



2684 



2685 



2686 



2687 



2688 



2689 



2690 

 2691 



2692 



2693 



Vaccinated Octo- 

 ber 27 subeutane- 

 ously with — 



One 48-hour cul- 

 ture killed 

 "Pest Viru- 

 lent." 



_do. 

 _do. 

 _do. 



.do. 



-do. 

 _do. 

 _do. 

 _do. 

 _do. 

 -do. 



-do — 



_do ... 



_do. 

 _do. 



Reinoculated 



November 26 



with — 



5 oesen suspen- 

 sion of "Pest 

 Virulent" 

 rubbed over 

 abdomen. 



do 



do 



do 



-do. 



-do. 



-do. 



__do. 



__do. 



__do. 



_-do. 



,_do. 

 -do. 



-do. 

 -do. 



Result. 



Alive and well 



do 



do 



Dead Dec. 4, 



after 8 days. 

 Dead Dec. 12, 



after 16 days. 

 Dead Dec. 5, 



after 9 days. 

 Dead Dec. 10, 



after 14 days. 

 Dead Dec. 13, 



after 17 days. 

 Dead Dec. 10, 



after 14 days. 

 Dead Dec. 4, 



after 8 days. 

 Dead Dec. 10, 



after 14 days. 

 Alive and well 

 Dead Dec. 8, 



after 12 days. 

 Dead Dec. 4, 



after 8 days. 

 Dead Dec. 7, 



after 11 days. 



Autopsy and remarks. 



Numerous pest bacilli in 



smears from spleen. 

 Large bubo. Typical pest 



spleen. 

 Typical pest bubo and spleen. 



Do. 



Multiple buboes. Infarcts in 



lung and spleen. 

 Typical bubo and spleen. 



Do. 



Do. 



Do. 



Do. 

 Do. 



