﻿200 



STRONG. 



Series 11. — Inoculations with "Pest Avirulent" in monkeys. 



Ten 24-hour agar slant cultures ( — 1 per cent alkaline to phenol phthaleln)* 

 of the strain "Pest Avirulent" were suspended each in 1 cubic centimeter of saline 

 solution, the whole 10 cubic centimeters mixed and 1 cubic centimeter, or about 

 1 agar slant, injected into each animal subeutaneously near the root of the tail. 

 Eleven days later the immunity of the animals was tested in the following 

 manner: Two 48-hour agar cultures of "Pest Virulent" (monkey number 1295, 

 second transplant) were suspended each in 5 cubic centimeters of bouillon, and 

 each animal was reinoeulated subeutaneously on the opposite side of the body 

 with 1 cubic centimeter of this suspension, equal to 2 oesen. 



Animal 

 No. 



Vaccination 

 October 19. 



Reinoeulated 

 October 30. 



Result. 



Autopsy and remarks. 



1277 



1 agar cul- 



2 oesen "Pest 



Dead Nov. 6 



Smears from the spleen revealed only a few 





ture. 



Virulent." 



(7 days). 



swollen bacilli. In smears from the heart 

 no organisms were found. In smears from 

 the tissues at the point of inoculation the 

 organisms are numerous. Cultures from 

 the heart's blood (2 cultures) negative; 

 plenty of blood inoculated. f 



1278 



do 



_ do 



Alive and 

 well. 









1279 



. . do 



do . . . 



Dead Nov. 2 

 (3 days). 



Innumerable pest bacilli in smears from the 

 spleen. 



1280 



do. — 



do 



Dead Nov. 6 

 (7 days). 



Smears from the spleen show only a few 

 degenerating bacilli. Those from the 















heart show no organisms. Those from the 











bubo near point of inoculation show 











numerous pest bacilli. Cultures from the 











heart reveal 6 colonies only of pest bacilli. t 



1281 



do 



do 



Dead Nov. 7 

 (8 days). 



Very numerous pest bacilli in smears from 

 the spleen. 



CONTROLS. 



1325 



None . .„ 



2 oesen "Pest 



Dead Nov. 2 



Innumerable pest bacilli in smears from the 







Virulent." 



(3 days). 



spleen. 



1326 



__. do 



do _ _ _ 



Dead Nov. 1 

 (2 days). 



Innumerable pest bacilli in smears from the 

 spleen. Culture from the heart=rich 















growth. 



1327 



do _.. .. 



do 



Dead Nov. 2 

 (3 days). 



Innumerable pest bacilli in smears from 

 spleen. 



1328 

 1329 



do 



__ do 



__. _do 



Dead Nov. 3 



(4 days). 



Do. 

 Do. 



do _ . 



do. . - 







*The growth of the pest bacilli on this agar appeared lighter than it did on + 1 per cent acid agar 

 which was used in all the other experiments. 



t The blood of these animals has apparently acquired considerable anti-infectious power, if one 

 may judge from the distribution of the bacilli. 



