﻿STUDIES IN PLAGUE IMMUNITY. 



201 



Series 12. — Inoculations with "Pest Avirulent" in monkeys. 



Thirteen 24-hour agar slant cultures of "Pest Avirulent" were suspended each 

 in 1 cubic centimeter of bouillon, the suspensions were mixed and monkeys num- 

 bered 1298 to 1301 inclusive were each inoculated subcutaneously with 2 cubic 

 centimeters and monkeys numbered 1302 to 1306 inclusive each with 1 cubic- 

 centimeter. Ten days after the vaccination the immunity of each animal was 

 tested in the following manner : Two agar slant 48-hour cultures of "Pest 

 Virulent" (from guinea pig number 1324, second transplant) were each suspended 

 in 5 cubic centimeters of bouillon and animals numbered 1298 and 1300 to 1305, 

 and controls numbered 1414 to 1422 were each inoculated subcutaneously with 

 0.25 cubic centimeter of this suspension, equal to i oese "Pest Virulent;" and 

 animal number 1306, with 0.5 cubic centimeter, equal to 1 oese. 



Animal 

 No. 



Vaccination 

 October 25. 



Reinoculated 

 November 10. 



Result. 



Autopsy and remarks. 



1298 



2 agar slants 



A oese "Pest 



Dead Nov. 22 



No pest bacilli in smears from spleen. 





"Pest Avir- 



Virulent." 



(12 days). 



Large suppurating bubo about point of 





ulent." 







inoculation containing pest bacilli and 

 many cocci and other bacilli. 



1299 



do 







Found dead about 12 hours after vacci- 











nation. There is a large suppurating 











wound over the abdomen. Cultures 











from this area developed colonies which 











were later identified as those of staphy- 











lococci and streptococci and of a colon 











bacillus. Cultures from the spleen 











show many staphylococci. Cultures 











near the point of inoculation show a 











few colonies of "Pest Avirulent" which 











have evidently survived. 



1300 



do 



J oese "Pest 

 Virulent." 



Alive and 

 well. 





1301 

 1302 



_do 



._ do 



„ do- . _ 





1 agar slant 

 "Pest Avir- 



.do 



do 













ulent." 









1303 



do 



___ do 



Dead Nov. 20 



No bacilli found in smears from spleen. 









(10 days). 



Suppurating bubo about point of inocu- 

 lation containing pest bacilli. 



1304 



do 



do __ 



Dead Nov. 16 

 (6 days). 



Very numerous pest bacilli in smears 

 from spleen. 







1305 



do 



do 



Dead Nov. 17 



No organisms found in smears from 









(7 days). 



spleen. Cultures from the heart, neg- 

 ative. Bubo at point of inoculation 

 comparatively small. A number of pest 

 bacilli present. 



1306 



do 



1 oese "Pest 



Alive and 









Virulent." 



well. 





