﻿202 



STRONG. 



Series 12 — Continued. 



CONTROLS. 



Anmial Vaccination 

 No. October 25. 



Reinoculated 

 November 10. 



Result. 



Autopsy and remarks. 



1414 

 1415 

 1416 



1417 



1418 



1419 

 1420 



1421 

 1422 



None.. 



do. 



do. 



_do. 

 -do. 



.do. 

 _do. 



_do. 

 .do. 



i oese "Pest 



Virulent." 

 do 



do 



_do_„ 

 .do 



-do. 



-do. 



_do. 

 -do. 



Dead Nov. 20 



(10 days). 

 Dead Nov. 13 



(3 days). 

 Dead Nov. 14 



(4 days). 



do 



Dead Nov. 15 



(5 days). 

 Dead Nov. 14 



(4 days). 

 Dead Nov. 13 



(3 days). 



_do. 



Dead Nov. 



14. 



Few pest bacilli in smears from spleen. 



Chronic bubo. 

 Innumerable pest bacilli in smears from 



spleen. 



Countless pest bacilli in smears from 



spleen. 



Do. 



Do. 



Do. 



Only comparatively few pest bacilli in 

 smears from spleen. (Edematous infil- 

 tration and countless numbers near 

 point of inoculation. 



Very numerous pest bacilli in smears 

 from spleen. 



Countless pest bacilli in smears from 

 spleen. Hfemorrhagic infarction of the 

 intestine. 



Series 18. — Inoculations with "Pest Avirulent" in monkeys. 



On November 6, twenty 48-hour agar cultures of "Pest Avirulent" were sus- 

 pended in 20 cubic centimeters of bouillon and each animal was vaccinated 

 with 2 cubic centimeters of this suspension, equal to 2 agar slant cultures. On 

 November 23, seventeen days after the first inoculation, two 48-hour agar slant 

 cultures of "Pest Virulent" (from guinea pig number 1452, second transplant) 

 were suspended in 10 cubic centimeters of bouillon and each animal was rein- 

 oculated subeutaneously, on the opposite side of the body to that upon which 

 the first inoculation was performed, with 0.25 cubic centimeter of this suspension, 

 equal to J oese. Ten control animals were also inoculated at the same time with 

 the same dose. 



Animal 

 No. 



Vaccinated 

 November 6 

 subcutane- 

 ously with — 



Reinoculated 



November 23 



with — 



Result. 



Autopsy and remarks. 



1373 



1374 

 1375 

 1376 

 1377 



1378 



2 agar cul- 

 tures "Pest 

 Avirulent." 

 do 



i oese "Pest 

 Virulent." 



_ do — 



Dead Nov. 29, 

 after 6 days. 



Alive and well _ 

 do _ 



A few typical pest bacilli in smears 

 from spleen. 



Pest bacilli very numerous in smears 

 from spleen. 



do 



do 



do 



do „ 



— do 



do 



do 



Dead Nov. 29, 



after 6 days. 

 Alive and well _ 



do_— . 



— do 











