﻿STUDIES IN PLAGUE IMMUNITY. 



203 



Series 18 — Continued. 



Animal 



No. 



Vaccinated 

 November 6 

 subcutane- 

 ous] y with — 



Reinoculated 



November 23 



with — 



Result. 



Autopsy and remarks. 



1379 



2 agar cul- 



J oese "Pest 



Dead Dec. 6, 



A large sloughing ulcer 4 centimeters 





tures "Pest 



Virulent." 



after 13 days. 



in diameter about point of inocu- 



■ 



Avirulent." 







lation. No plague bacilli in cultures 

 and smears from the spleen. Many 

 bacilli and cocci in smears from the 

 ulcer. The animal has evidently 

 died of plague intoxication, the 

 organisms multiplying locally. 



1380 



do 



do 



Dead Nov. 30, 



A fair number of pest bacilli in smears 

 from spleen. 









after 7 days. 



1381 



_ __do 



....do 



Dead Dec. 2, 



Only two involution forms of organ- 

 ism found in smears from spleen; 









after 9 days. 











there is a bubo about the point of 











inoculation in smears from which a 











number of pest bacilli are present. 



1382 



do 



____ do 



Alive and well . 









CONTROLS NUMBERS 1484 TO 1494. 



These controls constitute the same animals given in Series 17. They all died 

 of acute pest infection in from four to five days. For details of the autopsies 

 see Series 17 (p. 213). 



Series 51. — Inoculations with "Pest Avirulent" in monkeys. 



Twenty-five 48-hour agar slant cultures of the strain "Pest Avirulent" were 

 suspended in 25 cubic centimeters of saline solution ( 1 cubic centimeter to each 

 culture). Monkeys numbered 2706 to 2712 each received subcutaneously 2 cubic 

 centimeters of this suspension, equal to 2 agar cultures, and monkeys numbered 

 2713 to 2720 each received 1 cubic centimeter equal to 1 agar culture. On 

 November 28, one month after the vaccination, the immunity of the animals 

 was tested with a suspension of the strain "Pest Virulent" in exactly the same 

 manner as was the immunity of the animals comprising Series 48 and 52 (p. 217). 

 Twenty-five control animals, numbered 2896 to 2920, were also inoculated on this 

 date and with the same suspension. They all died of acute pest infection. For 

 the details of the protocols see Series 48 (p. 195). 



Animal 

 No. 



Vaccinated Octo- 

 ber 29 subcutane- 

 ously with — 



Reinoculated 

 November 28 

 subcutane- 

 ously with — 



Result. 



Autopsy and remarks. 



2706 



2707 

 2708 

 2709 



2710 



2711 

 2712 



Two 48-hour agar 

 cultures "Pest 

 Avirulent." 

 .do. 



I oese "Pest 

 Virulent." 



do 



Dead Dec. 1, 

 after 3 days. 



Alive and well - 

 . do. . 



Few pest bacilli in smears from 

 spleen. Numerous at point of 

 inoculation. 



Buboes with few pest bacilli. 



_ ..do 



do 



do 



do 



Dead Dec. 4, 



after 6 days 

 Alive and well . 



... do _ 



do. .. „ . 



do 



__ do 



.do . 



do 



do 



.do 









55670- 



