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STRONG. 

 Series 5 1 — Continued. 



Animal 

 No. 



Vaccinated Octo- 

 ber 29 subcutane- 

 ously with — 



Renoculated 

 November 28 

 subcutane- 

 ously with — 



Result. 



Autopsy and remarks. 



2713 



2714 

 2715 



2716 



2717 

 2718 

 2719 



2720 



One 48-hour agar 

 culture "Pest 

 A virulent." 



do . 



f oese "Pest 

 Virulent." 



.. .do. 



Dead Dec. 1, 

 after 3 days. 



Dead Dec. 2, 

 after 4 days. 



Dead Dec. 3, 

 after 5 days. 



Dead Dec. 1, 

 after 3 days. 



Alive and well _ 

 : do. .. 



Numerous pest bacilli in smears 

 from spleen. 



Do. 



Bubo and numerous pest bacilli in 



smears from spleen. 

 Numerous pest bacilli in smears 



from spleen. 



Numerous pest bacilli in smears 

 from spleen. 



—do . . . 



.do .. . 



do 



.do 



do 



...do 



.do 



do . 



do 



__do 



Dead Dec. 1, 



after 3 days. 



Alive and well _ 



. .do 



do 







Series 32. — Inoculations with "Pest Avirulent" in guinea pigs. 



May 16 : Twenty-four 48-hour agar slant cultures of "Pest Avirulent" were 

 suspended in 24 cubic centimeters of saline solution ( 1 cubic centimeter to each 

 culture) . Animals numbered 1975 to 1986, except number 1979, were each inoc- 

 ulated intraperitoneally with 2 cubic centimeters, equal to 2 agar cultures. The 

 guinea pigs comprising this series were small, averaging from 150 to 175 grams in 

 weight. On July 16, two months after the vaccination, the animals which 

 remained alive were all reinoculated in the following manner: A 48-hour agar 

 culture of "Pest Virulent" (from guinea pig number 2174, second transplant) 

 was suspended in 5 cubic centimeters of bouillon and 5 oesen of this suspension 

 rubbed over a small, freshly shaved area on the abdomen and the skin scarified 

 with a knife. Twenty-five controls were inoculated in the same manner and 

 served for this series and for the following one. ( Series 37 ) . 



Animal 

 No. 



Vaccinated 



intraperitoneally 



May 16 with— 



Reinoculated 

 July 16 with— 



Result. 



Autopsy and remarks. 



1975 



1976 

 1977 

 1978 



1980 



Two 48-hour cul- 

 tures "Pest 

 Avirulent." 



do 



5 oesen sus- 

 p e n sion 

 "Pest Viru- 

 lent." 

 .do - 



Alive and well . 

 ..do 



Numerous pest bacilli in smears 

 from spleen. Many swollen. 

 Few postmortem bacilli. 



do 



do . 



do 



do 



do. . 



Dead July 21, 

 after 5 days. 



Alive and well _ 



do _ . 



_ .do 







