﻿STUDIES IN PLAGUE IMMUNITY. 



207 



Series 37. — Inoculations with "Pest Avirule.nt" in guinea pigs. 



May 20: Ten 24-hour agar cultures of "Pest Avirulent" were suspended in 

 10 cubic centimeters of normal saline solution ( 1 cubic centimeter to each 

 culture). Each animal received 1 cubic centimeter intraperitoneally. Some of 

 the guinea pigs of this series did not weigh over 175 grams. One of these 

 apparently succumbed from the effect of the vaccination. On July 16, nearly 

 two months after the vaccination, the animals were all reinoculated with 5 oesen 

 of a suspension of "Pest Virulent" (one 48-hour culture from guinea pig number 

 2174, second transplant in 5 cubic centimeters of bouillon), rubbed over a freshly 

 shaved area on the abdomen which was scarified with a scalpel. These animals 

 were all inoculated in exactly the same manner and on the same day as the 

 animals comprising Series 32, 33 and 38 (pp. 218 and 219). Twenty-five control 

 animals, numbers 2098 to 2122, were also inoculated, all of which died. The 

 details of the autopsies are given in Series 32 (p. 206). 



Animal 



No. 



Immunized May 

 20 intraperito-* 

 nearly with— 



Reinoculated 

 July 16 with— 



Result. 



Autopsy and remarks. 



2036 

 2037 



2038 



2039 



2040- 



2041 



2042 



2043 



2044 



One 24-hour cul- 

 ture "Pest Avir- 

 ulent." 

 do 





Died June 1, 12 

 days after vac- 

 cination. 



Alive and well _.. 



Dead July 26, 

 after 10 days. 



Alive and well 



do 



No pest. 



Typical pest spleen and bubo. 

 Numerous pest bacilli in 

 smears from spleen. 



No post-mortem evidence of 

 pest. No peritonitis or 

 haemorrhages. Culture 

 from the abdomen devel- 

 oped from 150 to 200 colo- 

 nies. Evidently the ma- 

 jority of the organisms had 

 perished. Culture from 

 heart sterile. 



5 oesen suspen- 

 sion "Pest Vir- 

 ulent" over 

 abdomen. 

 do 



.do -• - - 



.do 



do - 



do 



do 



do 



.do.. 



do — 



_ __do __ 



___ do ._ - 



do.._ 



do .. 





Small guinea pig, 

 180 grams, died 

 May 21, less 

 than 24 hours 

 after vaccina- 

 tion. 



Alive and well 



do 



5 oesen suspen- 

 sion "Pest Vir- 

 ulent" over 

 abdomen. 





