﻿208 



STRONG. 



Series 39. — Inoculations with "Pest Avirulent" in guinea pigs. 



June 10: Fifteen 24-hour agar slant cultures of "Pest Avirulent" were sus- 

 pended in 15 cubic centimeters of normal saline solution, and each guinea pig 

 was inoculated subcutaneously with 1 cubic centimeter of the suspension. On 

 August 3, nearly two months after the vaccination, the animals were all rein- 

 oculated, 5 oesen of a suspension of ''Pest Virulent" (one 48-hour culture from 

 guinea pig number 2204, second transplant, in 5 cubic centimeters bouillon) 

 being rubbed over a shaved area on the abdomen which was scarified with a 

 knife. Twenty control guinea pigs were also inoculated at the same time and 

 in the same manner. These animals also served for controls for series number 

 40 (p. 219). 



Animal 

 No. 



Inoculated 

 subcutane- 

 ously June lu 

 with — 



Reinoculated 

 August 3 with — 



Result. 



Autopsy and remarks. 



2075 



One 24-hour 

 agar cul- 

 ture "Pest 

 Avirulent." 



5 oesen of suspen- 

 sion of "Pest 

 Virulent" rub- 

 bed over ab- 

 domen. 



Dead Aug. 11, after 8 days.. 



Pest. 



2076 



.__ do - 



_ _ do 



Dead Aug. 12, after 9 days__ 

 Alive and well 



Do. 



2077 

 2078 

 2079 



do___ ___ 



do.. 



Typical pest bubo. In- 

 numerable bacilli. 



do 



—do __ 



do 



_. do 



do 



Dead Aug. 10, after 7 days.. 









2080 

 2081 



_.do„ 



_ -do _ _ _ 



Alive and well 



Innumerable pest ba- 

 cilli in smears from 



do 



_ . do _ 



Dead Aug. 8, after 5 days 



















the spleen. 



2082 



___ do 



do 



Dead Aug. 11, after 8 days.. 



Alive and well . 



Dead Aug. 12, after 9 days.. 



Alive and well ... __ 



. do_ _ 



Do. 



2083 

 2084 



do 



.do- - 



Pest. 



_ _do 



.do. . 



2085 



2086 



2087* 



2088 



2089 



do — 



do 



Pest. Typical spleen, 

 containing necrotic 



do 



do . _ 



do .. 



do 



do . 



do J 



-do 



.do 



do- 



-do 



Dead Aug. 14, after 11 davs 



















foci. 



