﻿STUDIES IN PLAGUE IMMUNITY. 



Series 39 — Continued. 



209 



CONTROLS. 



Animal 

 No. 



Inoculated 



subcutane- 



ously June 10 



with — 



Reinoculated 

 August 3 with — 



Result. 



Autopsy and remarks. 



2249 



2250 

 2251 

 2252 

 2253 

 2254 

 2255 

 2256 

 2257 

 2258 

 2259 

 2260 

 2261 

 2262 

 2263 

 2264 

 2265 

 2266 

 2267 

 2268 



5 oesen of suspen- 

 sion of "Pest 

 Virulent" 

 rubbed over 

 abdomen. 



do 



Dead Aug. 12, after 9 days . 



-do. 

 _do. 

 _do. 

 -do. 

 _do. 

 _do. 

 .do. 

 _do. 

 _do. 

 .do. 

 _do. 

 _do. 

 _do. 

 -do. 

 -do. 

 _do. 

 _do_ 

 _do. 



Dead Aug. 

 Dead Aug. 

 Dead Aug. 

 Dead Aug. 

 Dead Aug. 

 Dead Aug. 

 Dead Aug. 

 Dead Aug. 

 Dead A tig. 

 Dead Aug. 

 Dead Aug. 

 Dead Aug. 

 Dead Aug. 



do— . 



Dead Aug. 



do_— 



Dead Aug. 

 Dead Aug. 

 Dead Aug. 



11, after 8 days -. 



13, after 10 days . 



10, after 7 days _. 



14, after 11 days-. 

 8, after 5 days__. 



11, after 8 days-. 



8, after 5 days___ 

 14, after 11 days . 

 11, after 8 days _. 

 7, after 4 days 

 10, after 7 days _. 



9, after 6 days ... 

 13, after 10 days_. 



11, after 8 days — 



10, after 7 days _ 

 9, after 6 days „ 

 13, after 10 days. 



Innumerable pest ba- 

 cilli in smears from 

 spleen. 



Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 



* Animal was pregnant at the time of vaccination and gave birth to two healthy young 7 days 

 after its reinoculation. 



Series 41. — Inoculations with "Pest Avirulent" in guinea pigs. 



June 30: Twenty-one 24-hour agar slant cultures of "Pest Avirulent" were 

 suspended in 21 cubic centimeters of saline solution and each guinea pig was 

 inoculated subcutaneously with 1 cubic centimeter of this suspension. On August 

 28, two months after the vaccination, the animals were all reinoculated with 

 5 oesen of a suspension of "Pest Virulent" (two 48-hour cultures from guinea 

 pig number 2315, second generation, in 10 cubic centimeters bouillon) being 

 rubbed over a freshly shaved and scarified area on the abdomen. Twenty-five 

 control animals were also inoculated at the same time and in the same manner. 



Animal 

 No. 



Vaccinated 

 June 30 subcu- 

 taneously 

 with — 



Reinoculated 



August 28 



with — 



Result. 



Autopsy and remarks. 



2146 



2147 

 2148 



2149 

 2150 



One 24-hour 

 culture 

 "Pest Avir- 

 ulent." 



„ .do 



5 oesen suspen- 

 sion of "Pest 

 Virulent" 

 rubbed over 

 abdomen. 

 do 



Alive and well 



Pest. 



Moderate-sized bubo 



and abscesses in the 



spleen. 



Dead Sept. 4, after 7 days __ 

 Dead Sept. 6, after 9 days _. 



Alive and well _ 



do 



do 



do 



do 



do 



do 



do 









