﻿STUDIES IN PLAGUE IMMUNITY. 



211 



Series 46. — Inoculations with "Pest Avirulent" in guinea pigs. 



October 26 : Fifteen 48-hour agar slant cultures of the strain "Pest Avirulent" 

 were suspended in 15 cubic centimeters of saline solution (1 cubic centimeter to 

 each culture). Each guinea pig of the series was inoculated subcutaneously with 

 1 cubic centimeter of this suspension. On November 26, one month after the 

 vaccination, the immunity of the animals was tested in the following manner: 



' Five oesen of a suspension of "Pest Virulent" (two 48-hour cultures from 

 guinea pig number 2858, second transplant in 10 cubic centimeters of bouillon) 

 being rubbed over a freshly shaved but not scarified area of the abdomen. 

 Twenty-five control animals were also inoculated at the same time and in the 

 same manner. The immunity of the animals of this series was tested on the 

 same date and with exactly the same suspension as was that of the animals 

 comprising Series 47, 50, and 54. (See pp. 220, 197, and 232.) 



Animal 

 No. 



Vaccinated 



October 26 sub- 



cutaneously 



with — 



Reinoculated 



November 26 



with — 



Result. 



Autopsy and remarks. 



2633 



2634 

 2635 



2636 

 2637 

 2638 

 2639 

 2640 

 2641 



2642 

 2643 

 2644 

 2645 

 2646 

 2647 



One 24-hour 

 culture 

 "Pest Avir- 

 ulent." 



do .. 



5 oesen suspen- 

 sion of "Pest 

 Virulent" 

 rubbed over 

 abdomen. 

 do 



Dead Dec. 4, after 8 days... 

 Alive and well . 



Typical pest buboes 

 and spleen. 



Old bubo and few ab- 

 scesses in spleen. 



Large bubo. Spleen 

 contains few ab- 

 scesses. 



do 



.do 



Dead Dec. 8, after 12 days __ 

 Alive and well 



.. do 



do 



do __ __ 



-do 



. .. do. 



do 



do 



do 



do 



do . - - 



do _ __ 



do 



do — 



do-. 



do 



.do 



do 



do 



Dead Dec. 3, after 7 days 



Alive and well 



_ _do__ 



do . - 



_ -do .. 



.... do 



do . 



. do — . 



do 



do 



do 



.do 



do 



do 



_ _ do . . 



- do - 



_ .do - - 









CONTROLS. 



2870 



2871 

 2872 

 2873 

 2874 

 2875 

 2876 

 2877 

 2878 

 2879 





5 oesen suspen- 

 sion of "Pest 

 Virulent" 

 rubbed over 

 abdomen, 

 do 



Dead Dec. 3, after 7 days 



Dead Dec. 1, after 5 days- 

 Dead Dec. 3, after 7 days.— 



Dead Dec. 2, after 6 days 



Dead Dec. 4, after 8 days- 

 Dead Dec. 1, after 5 days- 

 Dead Nov. 30, after 4 days— 

 Dead Dec. 2, after 6 days— 

 Dead Nov. 30, after 4 days.. 

 Dead Dec. 5, after 9 days 



Typical bubo. Numer- 

 ous pest bacilli in 

 smears from spleen. 



Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 

 Do. 







-do 





_. -do. - . - 





-do 





. do 





do — 





do 





do — 





do 







