﻿STUDIES IN PLAGUE IMMUNITY. 



Series 17 — Continued. 



213 



CONTROLS. 



Animal 

 No. 



Vaccinated Novem- 

 ber 3 subcutaneously 

 with — 



Infected Novem- 

 ber 23 with— 



Result. 



Autopsy and remarks. 



1484 



1485 

 1486 

 1487 



1488 

 1489 

 1490 



1491 



1492 

 1493 

 1494 





s oese "Pest Viru- 

 lent." 

 do _ 



Dead Nov. 28, 

 after 5 days, 

 .do 



Innumerable pest bacilli in 

 smears from the spleen. 

 Do. 

 Do. 

 Do. 



Do. 

 Do. 

 Do. 



Do. 



Do. 

 Do. 

 Do. 







do 



do .. 





do _ ■ - 



Dead Nov. 27, 



after 4 days. 

 do _ 





.J._do__.\ 





_ .do - - ______ 



. ._do ... 





_ do__ _ 



Dead Nov. 28, 

 after 5 days. 



Dead Nov. 27, 



after 4 days. 

 __ .do .. 





_ _do. 





___.do 





do 



do . . . 





. ..do . . 



.do . 









Series 21. — Inoculations vpth the strain "Maassen Alt" in monkeys. 



November 14: Twenty-one 48-hour agar slant cultures of "Maassen Alt" were 

 suspended in 21 cubic centimeters of bouillon. Monkeys numbered 1438 to 1445 

 inclusive each received 2 cubic centimeters subcutaneously, which is equal to 2 agar 

 slant cultures, and monkeys numbered 1446 to 1449 inclusive, 1 cubic centimeter 

 subcutaneously, equal to 1 agar slant culture. On November 28, two weeks 

 after the vaccination, the animals were all reinoculated in the following manner. 

 Two agar slant cultures of "Pest Virulent" (from guinea pig number 1479, 

 second transplant) were suspended in 10 cubic centimeters of bouillon and each 

 animal was inoculated subcutaneously on the opposite side of the body to which 

 the vaccination had been made with 0.25 cubic centimeter of this suspension, 

 equal to J oese "Pest Virulent." Twelve control animals were all inoculated at 

 the same time with the same dose of this suspension, except animals numbered 

 1503 and 1504, which were very large monkeys and which received 0.33 cubic 

 centimeter. 



Animal 

 No. 



Vaccinated 

 November 14 

 subcutane- 

 ously with— 



Infected 



November 28 

 with— 



Result. 



Autopsy and remarks. 



1438 



2 agar slant 

 cultures. 



J oese "Pest 

 Virulent." 



Alive and well . 





1439 



— do 



do 



Dead Dec. 5, 

 after 7 days. 



Very few pest bacilli in smears from 

 the spleen and near point of inocu- 















lation. Tissues near point of inocu- 











lation reddened and cedematous. 



1440 

 1441 

 1442 



_ .. do 



.do 



Alive and well _ 

 ..do. . — 



A few bacilli in smears from the 





do 



_—do — ... 



do 



Dead Dec. 6, 









after 8 days. 



spleen. Innumerable in ulcerating 

 bubo which also contains numerous 

 cocci. 



