﻿218 



STRONG. 



Seeies 33. — Inoculations with the strain "Maassen Alt" in guinea pigs. 



May 1(5: Twenty-two 48-hour agar slant cultures of the strain "Maassen Alt" 

 were suspended in 22 cubic centimeters of saline solution ( 1 cubic centimeter to 

 each culture). Guinea pigs numbered 1987 to 199(3 were inoculated intraperito- 

 neally each with 1 cubic centimeter. On July 16, two months after the vaccina- 

 tion, the animals were all reinoculated with the strain "Pest Virulent" in 

 exactly the same manner as the animals in Series 32. 37 and 38 (see pp. 204, 207 

 and 219), the same 25 control animals, numbered 2098 to 2122, all of which died 

 of pest, serving for all three of these series. 



Animal 

 No. 



Vaccinated 



intraperitone- 



ally May 16 



with — 



Infected 

 July 16 with— 



Result. 



Autopsy and remarks. 



1987 



1 cc. "Maas- 



5 oesen "Pest Viru- 



Alive and 







sen Alt.'' 



lent rubbed over 

 freshly shaved area 

 on abdomen and 

 skin lightly scari- 

 fied with a knife. 



well. 





1988 

 1989 

 1990 

 1991 

 1992 



do 



____ do, 



..do _ 



A few haemorrhages in the ab- 



_. do 



' do. 



.do. 



.do - - 



_do. ___ 



do 



do _ 



do_ - 



. _do 



. do .. 





Dead Mav 17, 









about 21 



dominal walls. No fluid. Cul- 









hours after 



ture from the abdominal cavi- 









vaccina- 



ty shows a fair number of pest 









tion. 



bacilli. 



1993 



do 



5 oesen "Pest Viru- 

 lent" rubbed over 

 freshly shaved area 

 on abdomen and 

 skin lightly scari- 

 fied with a knife. 



Alive and 

 well. 





1994 



do 





Dead Mav 17, 



No evidences of pest infection. 









23 hours af- 



Little fluid in the abdominal 









ter vaccina- 



cavity, culture from heart neg- 









tion. 



ative. In culture from ab- 

 dominal cavity a fair number 

 of pest bacilli developed. 



1995 



do 





Dead May 18, 

 2 days after 



Cultures from heart sterile. 

 Culture from abdomen, a rich 













vaccination. 



growth of pest. 



1996 



do. 



5 oesen "Pest Viru- 

 lent" rubbed over 

 freshly shaved area 

 on abdomen and 

 skin lightly scari- 

 fied with a knife. 



Alive and 

 well. 





