﻿220 



STRONG. 



Series 40 — Continued. 



Animal 

 No. 



Vaccinated 



subeutaneous- 



ly June 15 



with — 



Infected August 3 

 with — 



Result. 



Autopsy and remarks. 



2125 



One 24-hour 

 culture 



"Maassen 

 Alt." 



5 oesen of suspension 

 of "Pest Virulent" 

 rubbed over ab- 

 domen. 



Alive and well _ 





2126 



do 



do 



. __do 





2127 



do „ 



do 



do . . . . 





2128 



. do 





Dead June 16, 

 less than 24 



Culture from heart and spleen 

 negative. Cover slip from 













hours after 



point of inoculation shows a 









vaccination. 



few degenerating bacilli. 

 Animal evidently died of 

 plague toxaemia. 



2129 



do .. .. 



5 oesen of suspension 

 of "Pest Virulent" 

 rubbed over ab- 

 domen. 



Alive and well _ 





2130 

 2131 



__do 



do 



__do 



Few pest bacilli in smears from 



.do 



_ _ do . . 



Dead Aug. 14, 









after 11 days. 



spleen. 



2132 

 2133 



do 



do 



Alive and well . 

 Dead Aug. 12, 



Pest. 



do 



. do 









after 9 days. 





2134 

 2135 

 2136 



do 



do 



Alive and well . 

 —do. .. 





do 



.do 



do 



do 



do. .. 



2137 

 2138 



do „ 



.do 



do 



Pest. 



do 



.... do 



Dead Aug. 13, 









after 10 days. 





2139 



do 



do .. 



Alive and well _ 









Series 47. — Inoculations with the strain "Maassen Alt" in guinea pigs. 



October 26: Fifteen 48-hour agar slant cultures of the strain "Maassen Alt" 

 were suspended in 15 cubic centimeters of saline solution ( 1 cubic centimeter to 

 each culture). Each guinea pig of the series was inoculated subcutaneously with 

 1 cubic centimeter of the suspension equal to 1 agar slant culture. On November 

 26, about one month after the vaccination, the immunity of the animals was 

 tested in the following manner : 



Five oesen of a suspension of "Pest Virulent" (two 48-hour cultures from 

 guinea pig number 2858, second transplant, in 10 cubic centimeters of bouillon) 

 being rubbed over the freshly shaved but not scarified area of the abdomen. 

 Twenty-five control animals, numbered 2870 to 2894, were also inoculated with 

 the virulent organism at the same time and in the same manner. All of these 

 succumbed to acute plague infection. For the details of the protocols see 

 Series 46 (p. 211). 



