﻿STUDIES IN PLAGUE IMMUNITY. 

 Series 17 — Continued. 



221 



The immunity of the animals in this series was tested in the same manner 

 and with exactly the same suspension as was used for the animals comprising 

 Series. 46, 50, and 54. (See pp. 211, 197, and 232.) 



Animal 

 No. 



Vaccinated 



October 26 sub- 



cutaneously 



with— 



Infected 



November 26 



with — 



Result. 



Autopsy and remarks. 



2648 



One 24-hour 



5 oesen suspen- 



Alive and 







agar cul- 



sion of "Pest 



well. 







ture "Ma- 



"Virulent" rub- 









assen Alt." 



bed over abdo- 

 men. 







2649 

 2650 

 2651 

 2652 

 2653 



do 



do . 



do . 



No plague bacilli found in cover slips 

 from abdominal cavity or from point 



do 



do. 



do 



. .do 



.do - 



_ ..do 



do 



do _ 



do 



do 





Died Oct. 









27, 1 d a y 









after vac- 



of inoculation. Some fluid had en- 









cination. 



tered abdominal cavity. Animal 

 evidently died of toxaemia. 



2654 



.. .do 



5 oesen suspen- 

 sion of "Pest 

 Virulent" rub- 

 bed over abdo- 

 men. 



Alive and 

 well. 





2655 

 2656 



. .do 



. do 



do . .. 





do 



do 



do 



2657 

 2658 

 2659 

 2660 

 2661 

 2662 



do 



do .. . 



do 





. do. 



. .. do . _ 



do 



do 



do 



do . _ __ 



do _. 



do 



do. _ 



do 



. . do 



.do... 



do 



do 



do. 









EXPERIMENTS IN THE INOCULATION OF ANIMALS WITH EXTRACTS 

 OF THE STRAIN "PEST VIRULENT." 



Series 7. — Inoculations in monkeys with extracts of the strain "Pest Virulent" (free 



receptors). 



Twenty-one, 48-hour slant cultures of the strain "Pest Virulent" were sus- 

 pended in 21 cubic centimeters of saline solution. The organisms were killed 

 by heating for one hour at 60° C. and the suspension placed in the incubator 

 at 37° C. for three days. At the end of this time it was filtered through a 

 Berkefeld candle. The animals were inoculated subcutaneously with the filtrate 

 as shown below. Ten days later their immunity was tested by sticking them 

 with a 10 cubic centimeter syringe needle which had been dipped in a suspension 

 of "Pest Virulent" (one 48-hour agar slant "Pest Virulent," guinea pig number 

 1243, second transplant) suspended in 5 cubic centimeters of bouillon. 



