﻿222 



STRONG. 



Series 7 — Continued. 



Animal 

 No. 



How immun- 

 ized. 



How infected. 



Result. 



Autopsy and remarks. 



1242 



1245 

 1246 



1247 



5 cc. autoly- 



tic product 



= 50 mg. of 



culture. 



.do 



Stuck with 

 infected 10 

 cc. syringe 

 needle. 



_ _ do. 



Dead after 

 7 days. 



Dead after 



6 days. 

 Dead after 



11 days. 

 Alive. 



Innumerable pest bacilli in spleen. 



Pest septicaemia. Numerous organisms in 



the heart's blood. 

 Numerous organisms in the spleen. 



-do 



. ..do 



4. Scc.autoly- 

 tic product 

 = 48 mg. of 

 culture. 



do 



CONTROLS. 



1293 



1294 

 1295 





Stuck with 

 infected 10 

 cc. syringe 

 needle. 



do 



Dead after 

 4 days. 



Dead after 

 7 days. 



Dead after 

 4 days. 



Smears from spleen and heart negative for 

 organisms. Culture from heart. Staphy- 

 lococcus aureus. Pest organism prob- 

 ably still localized near point of inocu- 

 lation. 



No pest bacilli found in the smears from 

 the spleen. Very numerous in smears 

 from the tissues near the point of inocu- 

 lation. 



Innumerable pest bacilli in smears from 

 spleen. Cultures from heart show B. 

 pestis (pure). 







do. .. 







Series 26. — Inoculations in monkeys with extracts of the strain "Pest Virulent" 



{free receptors). 



On December 8 sixteen 48-hour agar slant cultures of "Pest Virulent" (from 

 guinea pig mimber 1514, second generation) were suspended in 16 cubic centi- 

 meters of distilled water, the organisms killed by heating at 60° C. for one hour 

 and the suspension then placed in the incubator at 37° C. for four days. At the 

 end of this time it was filtered through a Berkefeld candle. The suspension both 

 before and after filtration was sterile. On December 12 the animals of this 

 series were inoculated subcutaneously with the filtrate as below. On December 

 26, fourteen days after the first inoculation, the animals were infected in the 

 following manner: Six 48-hour agar slant cultures of "Pest Virulent" (from 

 guinea pig number 1592, second transplant) were suspended in 30 cubic centi- 

 meters of bouillon (5 cubic centimeters to each culture). Each animal was 

 inoculated subcutaneously on the opposite side of the body with 0.33 cubic 

 centimeters, equal to § oese, of "Pest Virulent." Twelve controls, numbered 1613 

 to 1624, were all inoculated at the same time with the same dose. The details 

 of the results with the controls may be seen in Table No. 25. (See p. 194.) 

 All of them succumbed to acute pest infection. 



