﻿STUDIES IN PLAGUE IMMUNITY. 



Series 26 — Continued. 



223 



Animal 

 No. 



Inoculated December 12 

 subcutaneously with — 



Reinoculation 



December 20 



with — 



Result. 



Autopsy and remarks. 



1583 



Free receptors extracted 



§ oese "Pest 



Dead Jan. 



Few pest bacilli in smears from 





from 5 agar slant cul- 



Virulent. 



13, after 



spleen. Innumerable organ- 





tures "Pest Virulent." 





17 days. 



isms in a large bubo over 

 which the skin is not yet 

 broken. 



1584 



do 



do 



Alive and 

 well. 









1585 



Free receptors extracted 



do 



Dead Jan. 



Numerous pest bacilli in 





from 2 agar slant cul- 





2, after 7 



smears from spleen. 





ture "Pest Virulent." 





days. 





1586 



Free receptors extracted 



do 



Dead Dec. 



Very numerous pest bacilli in 





from 1 agar slant cul- 





31, after 5 



smears from the spleen. 





ture "Pest Virulent." 





days. 





1587 



do 



_ _do 



Dead Jan. 1, 



Very few pest bacilli in smears 

 from the spleen. Organisms 









after 6 









days. 



very numerous in smears 

 from bubo. 



Series 29. — Inoculations with artificial pest aggressin in monkeys and guinea pigs. 



On January 27, large test tubes of agar were inoculated with first transplant 

 cultures of "Pest Virulent" from guinea pigs numbered 1700, 1698, 1697 and 1676, 

 all of which animals died between the 23d to the 25th of January. The cultures 

 were placed at 30° C. for two days. The growth was then suspended in distilled 

 water, 1 cubic centimeter being used for each culture containing about 30 milli- 

 grams of the growth. The whole was mixed and placed on an electrical shaking 

 machine for five days. The suspension was then heated for one and one-half 

 hours at a temperature of 44° to 45° C. and 5 per cent carbolic acid added in 

 sufficient quantity to form a 0.5 per cent solution. After one and one-half days 

 the suspension was thoroughly centrifuged for four hours at a velocity of 

 4,000 revolutions per minute and the clear fluid above pipetted and used for 

 the inoculations on February 7 as given below. Cultures showed this fluid to be 

 sterile. On February 24 and 25, nearly three weeks after the first inoculation, 

 the animals were reinoculated in the following manner: In the case of the guinea 

 pigs, the abdomen of each was freshly shaved, the skin scarified with a knife, 

 and a portion of the spleen of guinea pig number 1766, which had succumbed 

 this date to acute pest infection, was rubbed well over the scarified area. Three 

 control guinea pigs were inoculated in the same manner. The monkeys were 

 inoculated subcutaneously with 0.33 cubic centimeter of a suspension of "Pest 

 Virulent" (5 cubic centimeters of bouillon to one 48-hour agar slant culture 

 from guinea pig number 1764, second generation, equal to about § oese. 



Inoculations in guinea pigs. 



Animal 

 No. 



Inoculated Feb- 

 ruary 7 with — 



Infected February 

 24 with— 



Result. 



Autopsy and remarks. 



1731 

 1732 



5 cc. artificial ag- 

 gressin intra- 

 peritoneally. 



do 



Abdomen mas- 

 saged with por- 

 tion of pest 

 spleen. 

 do 



Dead Mar. 

 1, after 5 

 days. 



do - - 



Typical buboes containing nu- 

 merous pest bacilli. 



Do. 



