﻿STUDIES IN TLAGUE IMMUNITY. 



Series 42 — Continued. 



229 



Ani- 

 mal 

 No. 



Immunized 

 July 23 

 with— 



Second im- 

 munization 

 August 1. 



Infected August 24. 



Result. 



Autopsy and 

 remarks. 



2197 



5 ce. Artifi- 



5 cc. Artifi- 



Abdomen massaged with 



Dead Aug. 29, 



Numerous 





cial Ag- 



cial Ag- 



portions of the spleens of 



after 6 days. 



pest bacilli 





gressin 



gressin 



guinea pigs numbered 2315 





in smears 





IV. 



IV. 



and 2316 just dead of acute 

 plague infection. 





from 

 spleen. 



219S 



do 



do 



do _ 



Dead Sept. 2, 

 after 9 days. 



Subacute 

 pest. 









2199 

 2200 



do 



do— . 



do 



Alive and well . 

 do. __ __ 





do 



do 



do _ 

















CONTROLS. 







2322 







Abdomen massaged with 



Dead Aug. 29, 



Pest bacilli 









portions of the spleens of 



after 5 days. 



in smears 









guinea pigs numbered 2315 





from 









and 2316 just dead of acute 





spleen. 









plague infection. 







2323 







do .... 



Dead Aug. 31, 



Do. 











after 7 days. 





Series 34. — Animals inoculated for the preparation of natural plague aggressin. 



Animal No. 



Inoculated intraperitoneally with- 



Result. 



Abdominal exudate 

 collected. 



Rabbit 1966* 



Guinea pig 



1964. 

 Guinea pig 



1999. 



Guinea pig 



2000. 

 Guinea pig 



2001. 

 Guinea pig 



2002. 

 Guinea pig 



2003. 

 Guinea pig 



2004. 

 Guinea pig 



2007. 



Guinea pig 



2008. 

 Guinea pig 



2009. 

 Guinea pig 



2010. 

 Guinea pig 



2011. 



2 oesen of the heart's blood of guinea 



pig 1958 in 10 cc. bouillon on May 14. 

 Portion of spleen of guinea pig No. 1957 



shaken up in 5 cc. bouillon May 13. 

 J oese "Pest Virulent" 3 days' culture, 



first transplant from guinea pig No. 



1957 in 10 cc. bouillon May 17. 

 do 



.do. 

 .do. 

 _do. 

 .do. 



Dead May 20, 

 after 6 days. 



Dead May 14, 

 after 1 j days. 



Dead May 19, 

 after 2 days. 



Dead May 18, 

 after 1 day. 



Dead May 19, 

 after 2 days. 



Dead May 18, 

 after 1 day. 



do 



.do. 



oese 6 days' culture "Pest Virulent" 

 from guinea pig No. 1958 first trans- 

 plant in 10 cc. bouillon. 

 -_do 



Dead May 19, 

 after 1 day. 



do 



-do. 

 .do. 

 _do. 



Dead May 20, 

 after 2 days. 



Dead May 19, 

 after 1 day. 



Dead May 20, 

 after 2 days. 



2.75 cc. for Aggressin 



E. 

 5 cc. for Aggressin A. 



4 cc. for Aggressin C. 



4 cc. for Aggressin B. 



2 cc. for Aggressin C. 

 4.5cc. for Aggressin B. 

 4 cc. for Aggressin B. 



3 cc. for Aggressin B. 

 3 cc. for Aggressin C. 



1.75 cc. for Aggressin 



D. 

 4.5 cc. for Aggressin E. 



4.5 cc. for Aggressin 

 D. 



2 cc, foul, containing 

 many post-mortem 

 bacilli. Not used. 



* A number of rabbits were inoculated for the purpose of preparing natural aggressin. However, 

 these animals were found in Manila to be unsatisfactory for this purpose owing to the rapid con- 

 tamination of the fluid in the pleural or abdominal cavity due to post-mortem decomposition. 



