﻿230 



STRONG. 



Series 34 — Continued. 



The abdominal exudates from these guinea pigs were collected on the day of 

 the death of the animal and mixed with an equal volume of normal saline 

 solution. Five per cent carbolic acid was then added in sufficient amount 

 to form a 0.5 per cent solution. The mixtures were then heated for two hours 

 at 44° to 45° C. and subsequently centrifuged for four hours at a velocity of 

 4,000 revolutions per minute. The clear fluid above was then pipetted and 

 used in the inoculations described in Series 35. ' Its sterility was in each instance 

 demonstrated. 



Series 43. — Guinea pigs inoculated for the preparation of natural plague aggressin. 



Animal 

 No. 



Inoculated intraperitoneally with — 



Result. 



Exudate obtained. 



2201 



2202 



2203 

 2210 



2211 

 2212 

 2213 

 2214 

 2215 

 2216 

 2217 

 2218 

 2219 



1 oese "Pest Virulent" from guinea 

 pig No. 2179 first transplant in 10 

 cc. bouillon July 23. 

 _do ... _ 



Dead July 26, after 3 days.. 



Dead July 25, after 2 days.. 



Dead July 28, after 5 days __ 

 Dead July 31, after 1 day 



Dead Aug. 1, after 2 days __ 

 Dead July 31, after 1 day___ 

 do. 



5 cc. for Aggressin F. 



3.5 cc. foul exudate 

 obtained. Not 

 used. 



5 cc. for Aggiessin G. 



1 cc. for Aggressin H. 



3 cc. for Aggressin I. 



1 cc. for Aggressin H. 



2 cc. for Aggressin H. 

 1 cc. for Aggressin H. 

 1 cc. for Aggressin I. 



4 cc. for Aggressin H. 



Do. 

 Do. 

 2.5 cc. for Aggressin I. 



. .do . .. ... 



i oese 6-day culture of "Pest Viru- 

 lent" guinea pig No. 2183 first 

 transplant, in 5 cc. bouillon July 

 30. 

 do 



do _ _ 



do . . 



.do 



.___do _ _ 



do ___ 



Dead Aug. 1, after 2 days ._ 

 Dead July 31, after 1 day... 

 _ _do ___ _ 



do . 



do 



. do __ _. 



do _ ._ . .. 



do. 



Dead Aug. 1, after 2 days __ 





The abdominal exudates from these guinea pigs were collected on the day of 

 the death of the animal and mixed with an equal volume of normal saline solution. 

 Five per cent carbolic acid was then added, sufficient to form a 0.5 per cent 

 solution. The mixture was then heated for from one to two hours at a tem- 

 perature of 45° to 46° C. and subsequently centrifuged for four to five hours 

 at a velocity of 4,000 revolutions per minute. The clear fluid above was then 

 pipetted off and used in the inoculations described in Series 44. The sterility of 

 the aggressin was demonstrated in each instance. 



Series 35. — Inoculations with natural plague aggressin in guinea pigs. 



The animals were inoculated as below with natural aggressin, the preparation 

 of which is described in Series 34. On July 16 they were all, with the exception 

 of numbers 2053 and 2054 reinoculated by rubbing 5 oesen of a suspension of 

 "Pest Virulent" (5 cubic centimeters bouillon to one 48-hour culture guinea pig 

 numbered 2174, second transplant) over a shaved and lightly scarified area on the 

 abdomen. These animals were inoculated in exactly the same manner as those 

 given in Series 32 and 33 (see pp. 204 and 218), and the same twenty-five control 

 guinea pigs numbered 2098 to 2122 were employed for the present series. Guinea 

 pigs numbered 2053 and 2054 were inoculated June 18 by massaging a shaved area 

 over the abdomen with a portion of the spleen of gtiinea pig numbered 2124 and 

 scarifying the skin with a scalpel. Two control guinea pigs, numbered 2140 and 

 2141, were also inoculated in the same manner. 



