﻿232 



STRONG. 



Series 44. — Inoculations with natural plague aggressin in guinea pigs. 



The animals were inoculated intraperitoneal!^* July 27 to August 3 as given 

 below with natural aggressin, the preparation of which is described in Series 43 

 ( p. 230 ) . On August 28, about one month after the first inoculation, they were all 

 reinoculated, 5 oesen of a suspension of "Pest Virulent" (one 48-hour slant culture 

 from guinea pig numbered 2315 second transplant in 10 cubic centimeters bouillon) 

 being rubbed over a freshly shaved area of the abdomen of the animal and the 

 skin well scarified with a scalpel. Twenty-five control animals, numbered 2362 

 to 2386, were also inoculated in exactly the same manner and all died. The details 

 of these autopsies were given in Series 41 (p. 210). 



Animal 

 No. 



2205 



2206 

 2225 



2226 

 2232 



2233 

 2234 

 2235 

 2236 

 2237 

 2269 



2270 



Inoculated 

 intraperitone- 

 ally with— 



July 27, 4.5 cc. 

 Natural Ag- 

 gressin F. 



do 



July 30, 4.5 CC. 

 Natural Ag- 

 gressin G. 



do 



Aug. 1, 4 cc. 

 Natural Ag- 

 gressin H. 

 do 



_do. 



„do. 

 _do. 

 _do. 



Reinoculated 

 August 28 with- 



Aug. 3, 4 cc. 

 Natural Ag- 

 gressin I. 



do 



5 oesen suspen- 

 sion "Pest Viru- 

 lent" rubbed 

 on abdomen. 



do 



do 



.do. 

 .do. 



_do_ 



_do. 

 „do. 

 _do_ 

 _do. 

 _do. 



_do. 



Result. 



Dead Sept. 4, 

 after 7 days. 



Alive and well . 



Dead Sept. 15, 



after 18 days. 



Alive and well . 

 do 



Autopsy and remarks. 



_ | 



Dead Sept. 5, 



after 8 days. 

 Dead Sept. 8, 



after 11 days. 

 Dead Sept. 4, 



after 7 days 

 Dead Sept 



after 10 days. 

 Dead Sept, 4, 



after 7 days. 

 Alive and well 



Dead Sept. 9, 

 after 12 days. 



Numerous pest bacilli in smears 

 from spleen. 



Typical bubo. No organisms 

 found in smears from spleen. 



Fair numbers of pest bacilli in 



smears from spleen. 

 Pest bubo. One or two abscesses 



in spleen. 

 Numerous pest bacilli in smears 



from spleen. 

 Innumerable pest bacilli in smears 



from spleen. 

 Do. 



Innumerable pest bacilli in smears 

 from spleen. 



Series 54. — Inoculations with Klein's method in. guinea pigs. 



The buboes, lungs, spleen and liver containing necrotic foci were removed from 

 three guinea pigs which had died of subacute plague infection. The organs were 

 finely minced, spread out on glass in thin layers over sulphuric acid and dried at 

 from 46° to 47° C. for four days, and at 45° C. for two days longer. The dried 

 material was then rubbed up in a mortar with saline solution, the fluid portion 

 being decanted off from the sediment which did not dissolve. 



Each animal of the series received subcutaneously 2 cubic centimeters of this 

 suspension, equal to about 25 milligrams of the powder. In two of the animals, 

 numbered 2822 and 2823. large sloughs of the skin followed the inoculation. On 

 November 26, about one month after the injection, the immunity of the animals 

 was tested with the strain "Pest Virulent" in exactly the same manner as was 



