﻿STUDIES IN PLAGUE IMMUNITY. 



Series 54 — Continued. 



233 



that of the animals comprising Series 46, 47, and 50 (pp. 211, 220 and 197). 

 Twenty-five control animals, numbers 2870 to 2894, were also inoculated at the 

 same time and in the same manner. All succumbed from acute plague infection. 

 For the details of the protocols see Series 46. 



Animal 

 No. 



. Inoculated 

 October subcu- 

 taneously 

 with — 



Reinoculated 



November 26 



with — 



Result. 



Autopsy and remarks. 



2821 



2822 

 2823 

 2824 

 2825 

 2826 



2 cc. suspen- 

 sionofdried 

 plague or- 

 gans. 



do._ 



5 oesen suspen- 

 ■ sion "Pest Viru- 

 lent" rubbed 

 over abdomen. 

 do.. 



Dead Dec. 4-, after 8 days.— 

 do 



Typical bubo. Pest 

 bacilli in spleen. 



Do. 

 Do. 



Do. 



_do 



do 



Dead Dec. 5, after 9 days 



Alive and well - 



do 



. ..do... 



_ .do 



.do 



. do .. 



do 



do 



Dead Dec. 3, after 7 days 







Series 56. — Inoculations by Klein's method in guinea pigs. 



The buboes, lungs, spleen and liver containing necrotic foci were removed from 

 three guinea pigs, numbered 3017, 3018, and 3025 which had died of plague infection 

 and were used for the manufacture of the prophylactic employed in these experi- 

 ments. The prophylactic was prepared after the method of Klein as described in 

 Series 54 (p. 232). Each animal received 2 cubic centimeters of the suspension. 

 In three of the animals sloughs of the skin followed the inoculations. About one 

 month after the inoculations the immunity of the animals was tested by rubbing 

 5 oesen of a suspension of "Pest Virulent" (one 48-hour agar slant culture from 

 guinea pig number 3100, second transplant in 5 cubic centimeters bouillon) over 

 a shaved area on the abdomen. Three control animals inoculated in the same 

 manner and at the same time all succumbed to pest infection. 



Animal 



No. 



Inoculated subcuta- 

 neously with — 



Reinoculated March 12 

 with — 



Results. 



Autopsy 

 and re- 

 marks. 



3035 



3036 

 3037 

 3038 

 3040 

 3046 

 3047 



2 cc. suspension dried 

 plague aggressin. 



do 



5 oesen suspension "Pest 

 Virulent" rubbed over 

 abdomen. 



. ..do. __ _ 



Dead Mar. 16, after 4 days.. 

 Dead Mar. 20, after 8 days. 



Pest. 



Do. 



Pest. 

 Do. 



Pest. 



do 



do 



do ___ 



do._ _. __ . 



Dead Mar. 17, after 5 days— 

 Dead Mar. 16, after 4 days— 

 Alive and well 



do .. 



do 



.... do 



.do 



do . 



do 



Dead Mar. 18, after 6 days- 







CONTROLS. 



3052 



3055 

 3056 





5 oesen suspension "Pest 

 Virulent" rubbed over 

 abdomen, 

 .do 



Dead Mar. 17, after 5 days.. 



Dead Mar. 16, after 4 days— 

 Dead Mar. 16, after 4 days— 



Pest. 



Do. 

 Do. 







do 







