﻿246 STRONG. 



tested in dilutions of 1:20, 1:40, 1:80, 1: 160, 1:320; three controls of the 

 bacterial suspension without serum and two controls with normal serum in dilu- 

 tions of 1 : 10 and 1:40 were prepared. After one hour all reactions were 

 negative; after sixteen hours precipitation of the bacteria occurred in dilutions 

 of 1 : 20 and 1: 40 and in the normal serum in dilutions of 1: 10. The same 

 serum tested the following day; in dilutions of 1: 2, .gave a reaction after one and 

 one-half hours. 



Rabbit number 1967. — Two cubic centimeters of the autolyzed product obtained 

 from 20 milligrams of the strain "Pest Virulent" were injected intravenously; 

 the animal was killed ten days later and the agglutination in dilution of 1 : 2 

 tested. The reaction was negative after one and one-half hours. 



Rabbit number 1968. — Inoculated in the same manner as number 1967 and with 

 the same results. 



Monkey number 1278. — Inoculated subcutaneously October 19 with 1 agar 

 culture of "Pest Avirulent;" on October 30 inoculated subcutaneously with 2 

 oesen of "Pest Virulent." On November 17 the animal was killed and the agglu- 

 tination tested in dilutions of 1:2, 1:4, with two controls without serum. The 

 reactions were all negative after four hours. 



Monkey number 1285. — Inoculated October 20 by thrusting beneath the skin 

 a 5 cubic centimeter syringe needle dipped in a suspension of the strain "Pest 

 Virulent;" on October 25 the animal was reinoeulated with 2 oesen of "Pest 

 Virulent;" on November 17 it was killed and the agglutination tested in dilutions 

 of 1 : 2 and 1 : 4, with two controls of the bacterial suspension without serum. 

 All reactions were negative after two hours. 



Monkey number 1286. — October 20: The skin over the abdomen was scarified 

 and a suspension of "Pest Virulent" rubbed over this area. On October 25, 1 

 oese of "Pest Virulent" was injected subcutaneously; on December 2, § of an 

 agar slant of "Pest Virulent" was given subcutaneously; on December 14, § of 

 an agar slant of "Pest Virulent" was injected subcutaneously; on December 16 

 the animal died. The serum was tested on November 17 in dilutions of 1 : 2 and 

 1:4.- Both were negative after two hours. 



Monkey number 12S7. — Inoculated exactly as monkey number 1286 up to 

 December 14; the animal died on December 15, the day after the last inoculation. 

 The serum was tested on the following day in dilutions of 1 : 2 and 1:4; the 

 result was negative after two hours. 



Monkey number 1300. — October 25, two agar slant cultures of "Pest Avirulent" 

 injected subcutaneously; November 10, | oese "Pest Virulent;" on December 2, 

 2 oesen of "Pest Virulent" were given; on December 14, 4 oesen "Pest Virulent;" 

 on February 15, one 48-hour agar slant of "Pest Virulent" and on February 27, 

 two 48-hour agar slants "Pest Virulent" were injected. March 10 the aggluti- 

 nation was tested in dilutions of 1 : 2 and 1 : 10. After two hours there was 

 positive agglutination in dilutions of 1:2, the fluid being almost entirely clear. 

 A slight precipitation of the bacteria was observed in the dilution of 1: 10. 



Monkey number 1357. — November 2, infected with a 10 cubic centimeter syringe 

 needle dipped in a suspension of "Pest Virulent;" on December 2, A of an agar 

 slant of "Pest Virulent" was inoculated subcutaneously; on December 14, § of 

 an agar slant subcutaneously; on January 10, 1 agar slant of "Pest Virulent;" 

 on February 1, 14 agar slants of "Pest Virulent;" on February 27, 2 agar slants 

 of "Pest Virulent;" on March 15, 3 agar slants of "Pest Virulent." The animal 

 died on March 17. The agglutination in the dilutions of 1:5 was positive, the 

 fluid being clear after one hour; in the dilution of 1 : 10 there was a positive 

 weak reaction after four hours. 



Monkey number 2666. — Inoculated October 27. One 48-hour culture of killed 

 'Test Virulent" being given subcutaneously; on November 28, reinoeulated with 



