﻿248 



STRONG. 



Table No. III. — Normal serum of monkey number 3053 — Agglutination tests with 

 cultures grown for six generations in ice box and at 30° C. 



GROWN IN ICE BOX. 



Dilutions. 



After li hours. 



After 16 hours. 



1:10 

 1 :20 



{Control. 



IControl. 



Negative _ - 



Heavy precipitate. Fluid almost clear. 

 Do. 

 Do. 

 Do. 



■ -do 



__ do 



do . 



GROWN AT 30° C. 



1:10 

 1 :20 



fControl. 



IControl. 





Slight precipitate. Fluid not clear. 

 Do. 

 Do. 

 Do. 



_. _do _ 



__do 



do _ _ . . 





Guinea pig number 3031. — Inoculated subeutaneously January 20 with one-half 

 24-hour culture of "Pest Virulent" partially attenuated. The animal died 

 February 11, thirteen days after its inoculation, of advanced pest pneumonia and 

 pest bubo. Agglutinative values of the serum of this animal and of the serum of a 

 normal guinea pig (number 3053) were tested in dilutions of 1 : 5 and 1: 10; the 

 reactions were negative after two hours. After three hours, a moderate precipi- 

 tation occurred in all of the tubes; quite as marked in those without serum as 

 in the tubes with normal serum and in those of the pest guinea pig serum. 



Guinea pig number 2636. — October 26, one culture of "Pest Avirulent" given 

 subeutaneously. 



Guinea, pig number 2648. — October 26, one culture "Pest Maassen Alt" given 

 subeutaneously. On November 26 both animals were reinoculated cutaneousli/ 

 with 5 oesen of a suspension of "Pest Virulent;" on January 17 they were 

 reinoculated cutaneously with a portion of the spleen of a guinea pig (number 

 2093) which had just died of pest; the abdomen being deeply scarified. On 

 February 7, both animals were killed and the agglutination of their sera, together 

 with normal guinea pig serum (number 30.53). tested in dilutions of 1:2 and 1: 4. 

 The reactions were all negative after one hour ; after sixteen hours a precipitation 

 of the bacteria in nearly all of the tubes occurred. 



Pest immune horse serum Number I purchased from Asia. This serum in 

 doses of 1 to 2 cubic centimeters protected against fatal pest infection about 

 72 per cent of the rats inoculated. (See Series 8, 10, and 20, pp. 274, 275 

 and 281.) The agglutination was tested with the strain "Pest Virulent" 

 (second transplant) in dilutions of 1:2. 1: 5. 1: 10, 1: 20; controls with normal 

 horse serum in dilutions of 1 : 2, 1 : 5, 1 : 10, and two controls with saline solution 

 without serum were also prepared. After three hours all the reactions were 

 negative; after sixteen hours sedimentation occurred in the majority of the tubes. 

 In none of the tubes was the sediment more marked than it was in one of the 

 control tubes of the bacteria without serum. 



Pest immune serum 'Number II (obtained from horse), its anti-infectious power 

 ( see Series 27 ) being somewhat lower than that of Serum Number I. The 

 agglutinative value was tested with tluee strains of pest bacilli of different 

 virulence; at the same time a normal monkey serum was tested for control 

 purposes. The results obtained are as follows: 



