﻿250 



STRONG. 



Pest immune serum Number III from a horse in the process of immu- 

 nization by Dr. Kuediger. The last injection before bleeding was with 

 nearly 10 agar cultures of "Pest Virulent" intravenously. The serum was 

 drawn and tested for agglutination fifteen days after this injection. A 

 horse's normal serum was also tested at the same time. The results are 

 as follows : 



Table No. VI. — Immune serum Number III and normal serum (for control) — January 

 23, agglutination with strain "Pest Virulent" second transplant, 24-hour cultures. 



IMMUNE SERUM NUMBER III. 



Dilution. 



After 1 hour. 



After 4 hours. 



After 20 hours. 



1:2 

 1:10 



Negative . . 

 Positive 



Positive _ __ . 



Positive. 

 Do. 



. _do _ 





NORMAL SERUM (FOR CONTROL). 



1:2 



1:10 



fControl. 



[Control. 



Negative 



do 



do 



Negative 



Negative. 



Very slight sediment. 



Positive. 



Negative. 



do 



._ do__ _ __ _ 



.do 



-do 







Table No. VII. 



January 25, the same sera as in Table No. VI again tested, with the following 

 results : 



IMMUNE SERUM NUMBER III. 



Dilution. 



After 1 hour. 



After 2 hours. 



After 16 hours. 



1:10 

 1:20 



Positive _ 

 Negative _ .. 



Positive. Marked 



precipitate. 

 Negative _ . 



Positive fluid cleared. 



Considerable sediment. Fluid cleared. 







NORMAL SERUM (FOR CONTROL). 



1:10 



1:20 

 fControl. 

 IControl. 



Negative 

 do.. 



Negative _ _ 



Considerable sediment. Fluid almost 

 cleared. 



Do. 

 Negative. Slight precipitate. 



Do. 



. .do . 



_ _do 



do 



.do _ 



do__ __ 







The same sera were tested with a 24-hour culture of the strain "Pest A virulent'' 

 on this date. The results were as follows: 



